Potential mechanism of cisplatin resistance in non-small cell lung cancer A549 cells analyzed by the whole-transcriptome
- VernacularTitle:非小细胞肺癌细胞 A549 顺铂耐药潜在机制的全转录组测序分析
- Author:
Yaojun NI
1
,
2
,
3
,
4
,
5
;
Zhongneng XU
2
,
3
,
4
,
6
;
Sheng CHEN
2
,
3
,
4
,
6
;
Jun WANG
7
Author Information
1. 1. Department of Cardiothoracic Surgery, Hospital Affiliated to Nanjing Medical University and Huai&rsquo
2. an First People&rsquo
3. s Hospital, Huai&rsquo
4. an, 223001, Jiangsu, P.R.China
5. 2. Department of Cardiothoracic Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, P.R.China
6. Department of Cardiothoracic Surgery, Hospital Affiliated to Nanjing Medical University and Huai&rsquo
7. Department of Cardiothoracic Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, P.R.China
- Publication Type:Journal Article
- Keywords:
Non-small cell lung cancer;
cisplatin resistance;
whole transcriptome sequencing;
ceRNA network
- From:
Chinese Journal of Clinical Thoracic and Cardiovascular Surgery
2021;28(01):35-42
- CountryChina
- Language:Chinese
-
Abstract:
Objective To reveal the potential mechanism of cisplatin resistance in non-small cell lung cancer A549 cells by comparing the expression profiles of wild-type A549 cells and cisplatin-resistant A549 cells (A549/DPP) through whole transcriptome sequencing analysis. Methods The cisplatin resistant A549 (A549/DDP) cell line was first established. Then, the whole-transcriptome analysis was conducted both on A549 and A549/DDP cells. Next, the differentially expressed RNAs of lncRNA-seq, circRNA-seq, and miRNA-seq data were identified, respectively, followed by functional enrichment analysis. Finally, a comprehensive analysis based on the whole transcriptome data was performed and the construction of the ceRNA network was carried out. Results A total of 4 517 lncRNA, 123 circRNA, and 145 miRNA were differentially expressed in A549/DDP cells compared with the A549 cell line. These different RNAs were significantly enriched in cancer-related pathways. The ceRNA network contained 12 miRNAs, 4 circRNAs, 23 lncRNAs, and 9 mRNA nodes, of which hsa-miR-125a-5p and hsa-miR-125b-5p were important miRNAs based on the topological analysis. Conclusion Tumor necrosis factor signaling pathway and p53 signaling pathway are involved in A549/DPP resistance. Hsa-miR-125a-5p and hsa-miR-125b-5p may be potential targets for reversing cisplatin resistance.
