Study on the Effect and Its Mechanism of Bromophenylcurcumin on Apoptosis ，Migration and Invasion of Cho- langiocarcinoma Cells
- VernacularTitle:溴苯基姜黄素对胆管癌细胞凋亡、迁移和侵袭的影响及机制研究
- Author:
Ji’an ZHAO
1
;
Yanrong MA
2
;
Wenjia NIE
3
;
Liang DONG
3
;
Wencong LIU
4
;
Jingfeng GU
1
Author Information
1. Dept. of General Surgery，the First Hospital of Hebei Medical University，Shijiazhuang 050013，China
2. Dept. of Emergency，the First Hospital of Hebei Medical University，Shijiazhuang 050013，China
3. Dept. of Medical Service，the First Hospital of Hebei Medical University，Shijiazhuang 050013，China
4. Dept. of Ultrasonography，the First Hospital of Hebei Medical University，Shijiazhuang 050013，China
- Publication Type:Journal Article
- Keywords:
Bromophenylcurcumin;
Cholangiocarcinoma;
RBE cell;
JAK2/STAT3 signal pathway;
Migration;
Invasion
- From:
China Pharmacy
2021;32(4):467-474
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE：To study t he effects of bro mophenylcurcumin（GL63）on the apoptosis ，migration and invasion of human cholangiocarcinoma RBE cells ，and to investigate its mechanism based on JAK/STAT signaling pathway. METHODS ：MTT assay was used to detect the effects of different concentrations of GL 63 [0（blank control ，similarly hereinafter ），1.25，2.5，5，10， 20，40 μ mol/L] on the proliferation of RBE cells after 48 h treatment ；the IC 50 was calculated. The effects of different concentrations of GL 63（0，5，10，20 μmol/L）on colony formation were detected by crystal violet staining after 48 h treatment. Flow cytometry ，Hoechst 33342 staining，cell scratch test and Transwell chamber invasion test were used to detect the effects of different concentrations of GL 63（0，5，10，20 μmol/L）on cell cycle distribution ，apoptosis，migration and invasion ability after 24 h treatment. Western blotting assay was adopted to detect the effects of different concentration of GL 63（0，5，10，20 μmol/L） on the expression of JAK 2/STAT3 signal pathway associated proteins. RESULTS ：The proliferation inhibition rates of RBE cells in different concentrations of GL 63 groups（1.25-40 μmol/L）were significantly increase d，compared with blank control group （P＜ 0.01），and showed a dose-dependent trend ，with IC 50 of （8.46±1.30）μmol/L. Compared with blank control group， 85917439。E-mail：zhaoji-an-88@163.com inhibition rates of RBE cell colony formation were significantly decreased in different concentrations （5，10，20 μmol/L）of GL 63 groups（P＜0.01）. The percentage of RBE cells at G 0/G1 phase increased significantly ，while that at S phase decreased significantly （P＜0.01）. The apoptotic rate increased significantly（P＜0.01），and the nucleus showed dense pyknosis and apoptotic bodies. The rate of cell migration and healing was significantly decreased （P＜0.01），and the number of invasive cells through basement membrane was significantly decreased （P＜ 0.01）. The protein expression of p-JAK 2， p-STAT3， Bcl-2， MMP-2， MMP-9， Pro-caspase-9 and P ro-caspase-3 were down-regulated significantly while the expression of Bax ，Cyt-c，Cleaved-caspase-9 and Cleaved-caspase- 3 were up-regulated significantly（P＜0.01）. CONCLUSIONS ：GL63 may inhibit the proliferation ，migration and invasion of RBE cells and promote its apoptosis by inhibiting JAK 2/STAT3 signal pathway.
