Effect of Polyphyllin Ⅰ on Transcriptional Factor FOXQ1 and Epithelial-mesenchymal Transition in Colorectal Cancer Cell HCT116
10.13422/j.cnki.syfjx.20201121
- VernacularTitle:重楼皂苷Ⅰ对结肠癌HCT116细胞FOXQ1及上皮间质转化的影响
- Author:
Yan LUO
1
;
Yi-lan JIANG
1
;
Yong-min LI
1
;
Xiao-ning TAN
1
;
Jia-qin LIU
2
;
Yuan LYU
1
;
Ji LUO
1
Author Information
1. Hunan Academy of Traditional Chinese Medicine Affiliated Hospital, Changsha 410006, China
2. Graduate School, Hunan University of Chinese Medicine, Changsha 410208, China
- Publication Type:Research Article
- Keywords:
polyphyllin Ⅰ;
colon cancer;
HCT116 cells;
forkhead box Q1（FOXQ1）;
epithelial-mesenchymal transition (EMT)
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(11):119-123
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effect of polyphyllin Ⅰ on the expressions of forkhead box Q1（FOXQ1）and epithelial-mesenchymal transition (EMT)-related factors, in order to explore the possible mechanism of polyphyllin Ⅰ in inhibiting the metastasis of colon cancer. Method:After the treatment with 1.25，2.50 μmol·L-1 polyphyllin Ⅰ on HCT116 cells, Western blot and Real-time PCR were used to detect the expressions of FOXQ1，E-cadherin，Vimentin protein and mRNA. Result:Compared with the blank group, relative expressions of FOXQ1 protein and mRNA in low-concentration polyphyllin Ⅰ group were decreased, while relative expression of E-cadherin mRNA was increased, the differences were not statistically significant, and relative expressions of Vimentin protein and mRNA in low-concentration polyphyllin Ⅰ group were decreased (P<0.05)， and relative expression of E-cadherin protein in low-concentration polyphyllin Ⅰ group was increased (P<0.01). Compared with the blank group, relative expressions of FOXQ1, Vimentin protein and mRNA in high-concentration polyphyllin Ⅰ group were decreased，while relative expressions of E-cadherin protein and mRNA were increased (P<0.05, P<0.01). Compared with low-concentration polyphyllin Ⅰ group, relative expressions of Vimentin protein and mRNA in high-concentration polyphyllin Ⅰ group were decreased, but the difference was not statistically significant, relative expressions of E-cadherin protein and mRNA in high-concentration polyphyllin Ⅰ group were increased, whereas relative expressions of FOXQ1 protein and mRNA were decreased (P<0.05, P<0.01). Conclusion:Mechanism of polyphyllin Ⅰ inhibiting the metastasis of colon cancer may be related to the decrease of FOXQ1 and Vimentin expressions, and the up-regulation of E-cadherin.
