Analysis of Grading Evaluation Standard of Ophiopogonis Radix from Sichuan Province
10.13422/j.cnki.syfjx.20200147
- VernacularTitle:川麦冬药材的等级评价标准分析
- Author:
Hui WANG
1
;
Kun CHANG
1
;
Peng GAO
1
;
Song-bo QU
1
;
Yuan ZHANG
2
;
Ming-bo ZHAO
1
;
Chuan-kun SHEN
3
;
Zhi-yong LE
4
;
Yong JIANG
1
;
Peng-fei TU
1
;
Xiao-yu GUO
1
Author Information
1. State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China
2. School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China
3. Sichuan Daidai Weiben Agricultural Science and Technology Co.Ltd., Mianyang 621100, China
4. Kangmei Pharmaceutical Co.Ltd., Puning 515300, China
- Publication Type:Research Article
- Keywords:
Ophiopogonis Radix;
quality standard;
grading evaluation;
saponins;
homoisoflavonoids;
appearance;
paclobutrazol
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(9):194-201
- CountryChina
- Language:Chinese
-
Abstract:
Objective::To investigate the correlation between the characteristics and internal quality of Ophiopogonis Radix from Sichuan province through various quality evaluation, and provide reference for the formulation of grading standard of this herb. Method::For 28 batches of Ophiopogonis Radix, the characters, microscopic characteristics, thin-layer chromatography (TLC), the content of moisture, ash content, acid insoluble ash content, residues of sulfur dioxide, heavy metals, hazardous elements and paclobutrazol, water-soluble extract, contents of total saponins and three major components [ophiopogonin D (OPD), methylophiopogonanone A (MPOA) and methylophiopogonanone B (MPOB)] were determined.The relationships between various indicators and the grade of Ophiopogonis Radix were analyzed. Result::Ophiopogonis Radix exhibited specific properties in characters, microscopic characteristics and TLC.The contents of impurity, moisture, total ash, acid-insoluble ash and water-soluble extract existed differences in four grades of Ophiopogonis Radix from Sichuan province.For grade Ⅰ, grain number was 80-120 grains per 50 g, the moisture content was 11.1%-14.9%, total ash content was 1.6%-2.1%, acid-insoluble ash content was 0.03%-0.14%, water-soluble extract content was 77.0%-86.5%.For grade Ⅱ, grain number was 120-160 grains per 50 g, the moisture content was 13.1%-14.2%, total ash content was 1.3%-2.2%, acid-insoluble ash content was 0.06%-0.22%, water-soluble extract content was 75.9%-83.3%.For grade Ⅲ, grain number was 160-300 grains per 50 g, the impurity was 0.2%-8.4%, the moisture content was 12.6%-14.0%, total ash content was 1.2%-1.5%, acid-insoluble ash content was 0.06%-0.22%, water-soluble extract content was 74.0%-86.5%.For grade Ⅳ, grain number was 80-300 grains per 50 g, impurity was 1.2%-22.6%, the moisture content was 13.0%-15.4%, the total ash content was 1.4%-2.0%, acid-insoluble ash content was 0.03%-0.15%, water-soluble extract content was 79.8%-85.2%. Conclusion::It is reasonable and feasible to classify the grade of Ophiopogonis Radix from Sichuan province according to the appearance characteristics such as the grain number per 50 g and internal indexes such as impurity and water-soluble extract, which can be used as a reference for grading standards of Ophiopogonis Radix from Sichuan province.
