Identification of Chemical Constituents in Bufei Jianpi Formula by UPLC-Q-TOF-MS/MS
10.13422/j.cnki.syfjx.20200547
- VernacularTitle:UPLC-Q-TOF-MS/MS快速鉴定补肺健脾方的化学成分
- Author:
Lin-lin CUI
1
;
Yong-rui BAO
1
;
Shuai WANG
1
;
Tian-jiao LI
1
;
Xian-sheng MENG
1
Author Information
1. School of Pharmacy, Component Medicine Engineering Research Center of Liaoning Province, Liaoning Province Modern Chinese Medicine Research Engineerig Laboratory, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China
- Publication Type:Research Article
- Keywords:
Bufei Jianpi formula;
chemical constituents;
flavonoids;
alkaloids;
lignans;
organic acids;
chronic obstructive pulmonary disease
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(9):184-193
- CountryChina
- Language:Chinese
-
Abstract:
Objective::To identify and analyze the chemical constituents in Bufei Jianpi formula by UPLC-Q-TOF-MS/MS. Method::An Agilent Poroshell SB-C18 column (4.6 mm×100 mm, 2.7 μm) was used with a mobile phase system of 0.1% formic acid solution (A)-acetonitrile (B) for gradient elution (0-10 min, 3%B; 10-100 min, 3%-50%B; 100-120 min, 50%-100%B) under positive ion mode and water (A)-acetonitrile (B) for gradient elution (0-5 min, 3%B; 5-60 min, 3%-100%B) under negative ion mode, the flow rate was 0.6 mL·min-1, and the column temperature was 30 ℃. Mass spectrometric data were obtained under electrospray inoization (ESI) in positive and negative ion modes, the collection range was m/z 50-1 000.Agilent MassHunter Qualitative Analysis software was used to extract and match chromatographic peaks. Result::Combined with reference, related literature and database analysis, 95 compounds were identified by mass spectrometry information, including 41 flavonoids, 23 alkaloids, 12 lignans, 9 organic acids, and 10 other compounds. Conclusion::The chemical composition of Bufei Jianpi formula is complex, and the cracking rules of different components are different. Flavonoids are prone to deglycosylation, dehydration, Diels-Alder reaction (RDA) cleavage of the ring during lysis, and loss of some neutral molecules such as CO, CO2, CHO. Lignans has a substituent such as a hydroxyl group, a carbonyl group or a methoxy group on the benzene ring, and it is easy to obtain a fragment ion which loses H2O or CO. The basic structure of organic acids is a phenolic hydroxyl group-substituted aromatic ring, acrylic acid, fatty acid or the like, this kind of compound is easy to lose H2O and COOH in negative ion mode, and it is easy to break at the carbonyl to form fragment ions. This established method is rapid, sensitive and accurate, which can quickly identify the chemical constituents in Bufei Jianpi formula and provide evidences for clarifying efficacy material base of this formula.
