Chemical Constituents of Ginseng Radix et Rhizoma with Different Growth Years and Different Origins Based on LC-MS
10.13422/j.cnki.syfjx.20200315
- VernacularTitle:液质联用技术分析不同产地不同年限人参的化学成分
- Author:
Bo ZHANG
1
;
Xiu-li SUN
1
;
Yun-long GUO
1
;
Yang WANG
1
;
Shu-ying LIU
1
Author Information
1. Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, China
- Publication Type:Research Article
- Keywords:
Ginseng Radix et Rhizoma;
different origins;
different growth years;
3 years;
5 years
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(8):206-212
- CountryChina
- Language:Chinese
-
Abstract:
Objective::To study the components of Ginseng Radix et Rhizoma of different origins and growth years. Method::Rapid liquid chromatography-time of flight mass spectrometry (RRLC-Q-TOF-MS) was applied to detect the raw data of Ginseng Radix et Rhizoma.After peak extraction, alignment, and normalization, the multivariate statistical analysis was made for the resulted dataset to find out the different compounds.The compounds were identified by using accurate molecular weight and tandem mass spectra, and the standard references were used to further confirm the identification.The changing trends of these components in different ginseng samples were observed. Result::The ginseng samples of different growth years and different origins were divided into different groups in the score plot of PLS-DA, and the variables with the variable importance in projection (VIP) value of more than 1 were considered to contribute more to the separations, then the t-test was applied to determine whether potential biomarkers were statistically significant (P<0.05) between the two groups.The contents of eleven compounds, including ginsenoside Rb1, Rh4, and Rk2, were significantly different between ginseng samples aged 3 and 5 years, and the contents of these compounds increased as the rise of the ginseng growth years.Ginsenosides Rg1, Rf, Rh1, Rb1, and other six compounds were significantly different in ginseng samples from Jilin and Heilongjiang province. Conclusion::LC-MS is a rapid and accurate method for the analysis of ginseng samples, and could help to find out the different components among samples.
