Spectrum-effect Relationship of Anti-hepatoma Activity from 3 Kinds of Genus Paris Based on Cluster Analysis and Grey Relational Analysis
10.13422/j.cnki.syfjx.20192449
- VernacularTitle:基于灰色关联度分析和聚类分析的3种滇产重楼抗肝癌作用谱效关系探索
- Author:
Ruo-shi LI
1
;
Hui-qiong YUAN
1
;
Jiang LIU
1
;
Fei-ya ZHAO
1
;
Ai-en TAO
1
;
Bao-zhong DUAN
1
;
Ying WANG
1
;
Cong-long XIA
1
Author Information
1. College of Pharmacy and Chemistry, Key Laboratory of Universities in Yunnan Province on Development of Genuine Medicinal Materials Resources in Western Yunnan, Dali University, Dali 671000, China
- Publication Type:Research Article
- Keywords:
Paridis Rhizoma;
fingerprint;
anti-hepatoma effect;
cluster analysis;
grey correlation degree;
spectrum-effect relationship;
HepG2 cells
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(8):192-198
- CountryChina
- Language:Chinese
-
Abstract:
Objective::To study the spectrum-effect relationship between HPLC fingerprints and anti-hepatoma activity of Paris polyphylla var. yunnanensis, P. forrestii and P. vietnamensis, and to elucidate its effective substance. Method::HPLC was used to establish the fingerprint of three extracts from the plant. The mobile phase was consisted of acetonitrile (A)-water (B) for gradient elution (0-10 min, 20%A; 10-20 min, 20%-25%A; 20-30 min, 25%-30%A; 30-40 min, 30%-35%A; 40-50 min, 35%-40%A; 50-60 min, 40%A; 60-75 min, 40%-45%A; 75-80 min, 45%-60%A), and the flow rate was 0.9 mL·min-1. The UV detection wavelength was 203 nm. Thiazolyl blue tetrazolium bromide (MTT) array was used to detect the inhibitory effects of three extracts on the proliferation of HepG2 cells, and the half inhibitory concentration (IC50) was calculated. Cluster analysis and grey relational analysis were used to analyze the data of spectrum and efficacy, and to find out the components that contributed a lot to the anti-liver cancer effect. Result::A total of 11 common peaks were identified as common peaks among HPLC fingerprints of three kinds of Paris. After treated 72 h, P. forrestii has the highest inhibitory effect on the HepG2 cells, the IC50 of P. forrestii, P. polyphylla var. yunnanensis and P. vietnamensis were 148.33, 178.87, 208.09 mg·L-1, respectively. According to the grey relational analysis, the common peaks 1-10 from P. polyphylla var. yunnanensis had great correlation to anti-tumor effect, and the common peaks 1-7 for P. forrestii, the common peaks 1-4, 6-10, N1 for P. vietnamensis, all the correlation degrees with IC50 were >0.7.Cluster analysis of variables in each Paris showed that peaks with correlation degree >0.7 could cluster with IC50. Conclusion::The established HPLC fingerprint method is reliable with good reproducibility. The peaks 1-4, 6 and 7 from three kinds of Paris have the greatest contribution to the anti-hepatoma effect.
