DNA Barcoding Identification of Paecilomyces hepiali and Jinshuibao Capsules
10.13422/j.cnki.syfjx.20200948
- VernacularTitle:蝙蝠蛾拟青霉及金水宝胶囊的DNA条形码鉴定
- Author:
Xiao-hui ZHAO
1
;
Xia LIU
1
;
Cheng-zan ZHONG
2
;
Ming YANG
2
;
Le ZENG
2
;
Li XIANG
3
;
Shi-lin CHEN
3
Author Information
1. School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, China
2. Jiangxi Guoyao Pharmaceutical Co. Ltd., Nanchang 330052, China
3. Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
- Publication Type:Research Article
- Keywords:
Paecilomyces hepiali;
DNA barcoding;
internal transcribed spacer (ITS);
specific primers;
Cordyceps sinensis;
Jinshuibao capsules;
fermentation
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(8):156-162
- CountryChina
- Language:Chinese
-
Abstract:
Objective::Because traditional methods are difficult to identify the fermentation mycelium, DNA barcoding technology was used to quickly identify the raw material strain Paecilomyces hepiali of Jinshuibao capsules and related products. Method::A total of 168 samples of 8 species of P. hepiali and its confusable species were identified by internal transcribed spacer (ITS) sequences, and based on the ITS sequences, P. hepiali specific primers were designed to quickly identify the related products. Result::The length of ITS sequences in 44 P. hepiali samples from different sources was 499 bp and there was no mutation site. It was shown that P. hepiali could be distinguished from 7 confusable species based on ITS sequences. The specific primer (ITS-BF/ITS-BR) of P. hepiali designed by ITS sequences could be amplified to obtain a short fragment of 102 bp in length, which could be used to rapidly identify P. hepiali from other confusable species, and to distinguish relevant products in the market. Conclusion::The rapid identification of P. hepiali and its related products can be achieved through the ITS sequences and specific primers, which provides a reference for the production and quality control of Jinshuibao capsules.
