Effect of Testudinis Carapax et Plastrum Aqueous Extract Regulating NF-κB Inflammation Microenvironment on Osteogenic Differentiation of MC3T3-E1
10.13422/j.cnki.syfjx.20200838
- VernacularTitle:龟甲水提液调控NF-κB炎症微环境对MC3T3-E1成骨分化的影响
- Author:
Yu-zhuo ZHANG
1
;
Qi SHANG
1
;
Geng-yang SHEN
2
;
Hui REN
2
;
Hai-qing AO
1
;
Xiao-bing JIANG
2
;
Ben-gen ZHOU
2
Author Information
1. Guangzhou University of Chinese Medcine, Guangzhou 510006, China
2. The First Affiliated Hospital of Guangzhou University of Chinese Medcine, Guangzhou 510405, China
- Publication Type:Research Article
- Keywords:
Testudinis Carapax et Plastrum aqueous extract;
mouse preosteoblast cell line(MC3T3-E1);
osteogenic induction;
nuclear transcription factor-κB;
inflammatory microenvironment
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(8):89-94
- CountryChina
- Language:Chinese
-
Abstract:
Objective::To investigate the role and mechanism of Testudinis Carapax et Plastrum aqueous extract in promoting osteogenic differentiation of mouse preosteoblast cell line(MC3T3-E1) by regulating nuclear transcription factor-κB(NF-κB) inflammation microenvironment. Method::MC3T3-E1 cells were cultured in vitro, and osteogenic induction (OI) was performed. Testudinis Carapax et Plastrum was prepared and treated the cells. Cells were devided into control group, osteogenic induction group and Testudinis Carapax et Plastrum (20 mg·L-1)with osteogenic induction group. The proliferation of MC3T3-E1 was detected by cell counting kit-8 (CCK-8), and the optimum concentration of intervention was determined. MC3T3-E1 differentiation and osteogenic mineralization were assayed using alkaline phosphatase (ALP) and Alizarin red staining (ARS), respectively. The expressions of NF-κB p65, NF-κB p105, interleukin-6(IL-6), ALP and Collagen-Ⅰ(COL-Ⅰ) mRNA were detected by Real-time PCR. Result::The results of CCK-8 showed that the proliferation of MC3T3-E1 did not change statistically with time, but it showed an upward trend, while the proliferation at 20 mg·L-1 was more obvious than other groups. The ALP and ARS showed that the positive staining rate of osteogenic induction group and Testudinis Carapax et Plastrum with osteogenic induction group were higher than control group.Real-time PCR results showed that on the 7th day in culture, the expression of NF-κB p105 and IL-6 mRNA in Testudinis Carapax et Plastrum with osteogenic induction group was significantly lower than that in control group (P<0.01), and the expression of ALP and COL-Ⅰ mRNA was significantly upregulated(P<0.05), on the 14th day, the expression of NF-κB p65, NF-κB p105 and IL-6 mRNA in Testudinis Carapax et Plastrum with osteogenic induction group was significantly lower than that in control group (P<0.01). The expression of ALP and COL-Ⅰ mRNA was significantly increased (P<0.05, P<0.01). Conclusion::Testudinis Carapax et Plastrum aqueous extract can promote osteogenic differentiation of MC3T3-E1 via a mechanism associated with the regulation of inhibition of NF-κB inflammatory microenvironment.
