Effect of Yanghetang on Apoptosis of Triple Negative Breast Cancer Cell Line MDA-MB-231 Based on Egr1/p21 Signaling Pathway
10.13422/j.cnki.syfjx.20200424
- VernacularTitle:阳和汤对三阴性乳腺癌细胞MDA-MB-231凋亡Egr1/p21信号通路的影响
- Author:
Kang-le LI
1
;
Xiao-qian YANG
1
;
Yu ZHANG
2
;
Shuo YANG
3
;
Zhong-bo ZHU
3
;
Jian-wei DOU
1
Author Information
1. College of Pharmacy, Xi'an Jiaotong University, Xi'an 710061, China
2. The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, China
3. College of Basic Medical Sciences, Gansu University of Chinese Medicine, Lanzhou 730000, China
- Publication Type:Research Article
- Keywords:
triple negative breast cancer;
MDA-MB-231 cells;
Yanghetang;
early growth response gene 1 (Egr1);
cyclin dependent inhibitor (p21)
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(8):62-67
- CountryChina
- Language:Chinese
-
Abstract:
Objective::To investigate the effect of ancient recipe Yanghetang and its drug-contained serum on apoptosis of triple negative breast cancer cell line MDA-MB-231 based on the signal pathway of early growth response gene 1 (Egr1)/cyclin dependent inhibitor (p21). Method::The drug-containing serum of Yanghetang was prepared from rats, and MDA-MB-231 cells were cultured in vitro. The blank control group was set, and MDA-MB-231 cells in Yanghetang high, middle, and low dose groups (23.4, 11.7, 5.85 g·kg-1) were intervened for 24, 48, 72 h, respectively. After that, the cell counting kit-8(CCK-8) method was used to detect the cell proliferation of each group. The blank control group was set, while MDA-MB-231 cells in Yanghetang high, middle, and low dose groups (23.4, 11.7, 5.85 g·kg-1) were treated for 48 h, and then flow cytometry was used to detect the apoptosis of each group and the distribution of cell cycle. The expression of Egr1 and p21 mRNA in each group was detected by quantificational Real-time polymerase chain reaction (Real-time PCR), while the expression of Egr1 and p21 protein in each group was detected by Western blot. Result::After MDA-MB-231 cells were intervened by Yanghetang for 24, 48, 72 h, MDA-MB-231 cell proliferation was significantly inhibited in Yanghetang high and middle dose groups as compared with the blank control group (P<0.01). After MDA-MB-231 cells were intervened by Yanghetang for 48 h, the apoptosis was significantly increased in Yanghetang high and middle dose groups as compared with the blank control group (P<0.05, P<0.01). In the Yanghetang high, middle dose groups, the proportion of cell cycle G0/G1 phase decreased, and the proportion of G2/M phase increased (P<0.05, P<0.01). The mRNA and protein expressions of Egr1, p21 were increased in Yanghetang high and middle groups (P<0.05, P<0.01). Conclusion::Yanghetang can activate Egr1/p21 signaling pathway in MDA-MB-231 cells, increase the expression of Egr1 and p21, and cause G2/M cell cycle arrest, thereby inducing apoptosis of MDA-MB-231 cells.
