Establishment of a New Evaluation Method for Identification of Drug Resistance of Staphylococcus aureus Based on Rapid Identification System of Microorganisms
10.13422/j.cnki.syfjx.20201016
- VernacularTitle:基于微生物快速鉴定系统对金黄色葡萄球菌耐药性鉴定新评估方法的建立
- Author:
Tong-tong JIANG
1
;
Gui-jie TIAN
2
;
Xian-yu LI
3
;
Yi WANG
3
;
Yue-yi LI
1
;
Ya-jun XING
3
;
Ya-nan SUN
3
;
Shu-hua MA
3
;
Jin-he ZHOU
1
;
Wei-feng YANG
3
;
Zhong-mei HE
1
Author Information
1. Jilin Agricultural University, Changchun 130000, China
2. Siping Tiexi District Health and Health Bureau, Siping 136000, China
3. Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing 100700, China
- Publication Type:Research Article
- Keywords:
Staphylococcus aureus;
Pseudomonas aeruginosa;
methicillin-resistant Staphylococcus aureus;
microdilution method;
MALDI Biotyper System
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(10):128-135
- CountryChina
- Language:Chinese
-
Abstract:
Objective::To explore the feasibility of the rapid identification system(MALDI-Biotyper System) of microorganisms for rapid identification of Pseudomonas aeruginosa and clinical isolation of Staphylococcus aureus. Method::Identification quality control and clinical isolation were conducted for drug resistance of S. aureus by microbial rapid identification system and broth dilution method. The scores of microbial rapid identification system were compared with the MIC value of broth dilution method. The drug resistance of P. aeruginosa was simultaneously identified to determine the accuracy and applicability of the rapid identification system of microorganisms. Result::The scores of the microbial rapid identification system showed that the score of sensitive quality control strain S. aureus was higher than 2.000, and the that of resistant strain of methicillin-resistant S. aureus(methicillin-resistant S. aureus, MRSA)was between 1.700 and 2.000.The score of clinically isolated S. aureus was between 1.700 and 2.000, which suggested the drug resistance and was consistent with the MIC value of the broth dilution method. At the same time, the systemic identification value of the P. aeruginosa, which is independent of the quality control sensitive strain, was greater than 2.000, showing sensitivity and it was a sensitive strain itself, which was consistent with the results. Conclusion::The microbial rapid identification system scoring method can be used for the rapid identification of the drug resistance of S. aureus and P. aeruginosa.
