Effect of Strong Light Stress on Growth, Physiological and Biochemical and Gene Expression of Key Enzymes in Atractylodes lancea
10.13422/j.cnki.syfjx.20201011
- VernacularTitle:强光胁迫对茅苍术生长、生理生化及关键酶基因表达的影响
- Author:
Yu-zhuo WANG
1
;
Yu-chen GU
1
;
Jian-guo CHAO
1
;
Wei GU
1
;
Ye-long SHENG
2
;
Yun XIA
1
;
Kai WANG
1
Author Information
1. College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China
2. Changzhou Menghe Shuangfeng Chinese Herbal Medicine Technology Co. Ltd., Changzhou 213000, China
- Publication Type:Research Article
- Keywords:
Atractylodes lancea;
strong light stress;
3-hydroxy-3-methylglutaryl CoA reductase;
farnesyl pyrophosphate synthase gene
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(10):119-127
- CountryChina
- Language:Chinese
-
Abstract:
Objective::To explore the effect of strong light stress on the growth, physiological and biochemical and key enzyme gene expression of the Atractylodes lancea, in order to provide the scientific basis for the standardized cultivation of the A. lancea. Method::The two-year-old A. lancea seedlings were taken as experimental materials. Poplar forest (light transmittance between 18.26%-36.04%) was taken as control group(ck). Different density shading networks were used to simulate different degrees of high light stress (51.10%, 80.73%, 100%) in late July. The growth state of A. lancea was observed. On the 0th, 5th, 10th, 15th, 20th days, the physiological and biochemical indexes of malondialdehyde (MDA) content, cell membrane permeability, proline (Pro) content, antioxidant enzyme activity and chlorophyll content in the leaves of A. lancea were measured. The relative expression levels of 3-hydroxy-3-methylglutarate monoacyl coenzyme A reductase (3-hydroxy-3-methylglutaryl coenzyme A, HMGR) and farnesyl pyrophosphate synthase gene (farnesyl pyrophosphate synthase, FPPS) in leaves of A. lancea under intense light stress were determined by real-time fluorescence quantitative PCR(Real-time PCR). Result::After strong light stress, the color of the leaves of A. lancea changed from dark green to light green and yellowish green, and the burn of leaves became more and more serious. The contents of MDA, conductivity and Pro showed an upward trend with the increase of transmittance. Peroxidase (POD), superoxide dismutase (SOD) and catalase (CAT) tended to increase first and then decrease. The chlorophyll content decreased with the increase of light transmittance. The relative expression of HMGR in leaves of A. lancea decreased with the increase of light transmittance, while FPPS increased first and then decreased. Conclusion::The results showed that A. lanceaa could alleviate the inhibition of strong light stress by increasing the activity of antioxidant enzymes and regulating the content of osmotic pressure under certain strong light stress. Excessively strong intensity light stress leads to disequilibrium of metabolic mechanism of A. lancea, and seriously inhibits the plant growth.
