LCMS-IT-TOF Analysis of Retention Behavior of Sanguisorbae Radix Components on D101 Macroporous Resin and Polyamide Resin
10.13422/j.cnki.syfjx.20200847
- VernacularTitle:地榆成分在D101型大孔树脂和聚酰胺树脂上保留行为的LCMS-IT-TOF分析
- Author:
Miao-kai LYU
1
;
Zhi-peng HUO
2
;
Yuan-xue LIU
2
;
Yu-ting TIAN
1
;
Yu WANG
2
;
Chun-yong WU
1
;
Yi HE
2
Author Information
1. School of Pharmacy,China Pharmaceutical University,Nanjing 210009,China
2. Development Center of Modern Chinese Medicine,Research Institute of Tasly Holding Group Co.Ltd.,Tianjin 300410,China
- Publication Type:Research Article
- Keywords:
Sanguisorbae Radix;
aqueous extract;
macroporous resin;
liquid chromatography-ion trap-time-of-flight mass spectrometry (LCMS-IT-TOF);
chemical composition;
distribution rules;
polyamide resin
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(14):152-163
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study on the material basis of Sanguisorbae Radix by column chromatography and liquid chromatography-ion trap-time-of-flight mass spectrometry (LCMS-IT-TOF), and analyze the distribution of different components in Sanguisorbae Radix water extract on D101 macroporous resin and polyamide resin. Method:Sanguisorbae Radix water extract was separated by D101 macroporous resin and polyamide resin, and LCMS-IT-TOF was used for detection, chromatography separation was achieved on an ACQUITY UPLC HSS T3 column (2.1 mm×100 mm, 1.8 μm) with the mobile phase consisted of water (A) and acetonitrile (B) for gradient elution (0-10 min, 5%-20%B; 10-18 min, 20%-35%B; 18-23 min, 35%-50%B; 23-28 min, 50%-90%B; 28-30 min, 90%B; 30-33 min, 90%-5%B; 33-35 min, 5%B), the flow rate was 0.3 mL·min-1, the column temperature was 30 ℃. Data acquisition was carried out in electrospray ionization (ESI) under the positive and negative ion modes, the scanning range was m/z 100-1 200. According to mass spectrometry data such as accurate molecular mass and fragment information, combined with literature, different chemical components in loading effluents and ethanol eluents of Sanguisorbae Radix water extract were identified. A heat map of the distribution of components in each fraction was drawn by extracting mass spectrum peak intensity data of each sample. The elution rules of various components were compared visually. Result:The enrichment and separation of D101 macroporous resin and polyamide resin were obvious. Tannins in Sanguisorbae Radix water extract was mainly concentrated in loading effluent of macroporous resin and its water eluent, triterpenoids were mainly distributed in the 90% ethanol eluent of macroporous resin. In the above effluents and eluents, a total of 63 compounds (including isomers) were identified. Among them, 6 compounds, ellagic acid-4-pyranoarabinoside or its isomer, 6-O-galloylnorbergerin, 3-O-galloylnorbergerin, (6-acetyloxy-5,7-dihydroxy-8-methoxy-4-oxochromen-2-yl) acetate, ethyl 2-methyl-5,6-bis (sulfooxy) benzofuran-3- carboxylate were first discovered in Sanguisorbae Radix. Conclusion:The method can quickly and accurately identify the distribution of components in aqueous extract of Sanguisorbae Radix after column chromatography, providing experimental basis for exploring the pharmacodynamic components and mechanism of Sanguisorbae Radix.
