Analysis of Chemical Components in Supernatant and Precipitate of Sanajon Oral Liquid Based on UPLC-Q-TOF-MSE Technology
10.13422/j.cnki.syfjx.20200848
- VernacularTitle:基于UPLC-Q-TOF-MSE技术的三勒浆口服液上清液及沉淀部分化学成分分析
- Author:
Hao-zhou HUANG
1
;
Min QIU
1
;
Jin-fa TANG
2
;
Meng-Qi LI
3
;
Hai-yan LIU
4
;
San-hu FAN
4
;
Peng TAN
5
;
Li HAN
1
;
Jun-zhi LIN
6
;
Ding-kun ZHANG
1
Author Information
1. School of Pharmacy,State Key Laboratory of Characteristic Chinese Drug Resources in Southwest China,Chengdu University of Traditional Chinese Medicine (TCM),Chengdu 611137,China
2. The First Affiliated Hospital of Henan University of TCM,Zhengzhou 450000,China
3. Sichuan Nursing Vocational College,Chengdu 610100,China
4. Sanajon Pharmaceutical Group, Chengdu 610000,China
5. State Key Laboratory of Biological Evaluation of TCM Quality,National Administration of TCM,Sichuan Academy of Chinese Medicine Sciences,Chengdu 610041,China
6. Teaching Hospital of Chengdu University of TCM,Chengdu 610072,China
- Publication Type:Research Article
- Keywords:
Sanajon;
oral liquid;
precipitation;
polyphenols;
tannins;
ellagic acid;
phlobaphene
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(14):143-151
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the main chemical components in the supernatant and precipitate of Sanajon oral liquid, so as to provide basis for establishing its quality standard and precipitation control technology. Method:UPLC-Q-TOF-MSE was used to analyze the chemical components in the supernatant and precipitate of this oral liquid. The analysis was performed on Waters ACQUITY UPLC BEH C18 column (2.1 mm×100 mm, 1.7 μm) with the mobile phase of 0.1% formic acid solution (A) and acetonitrile (B) for gradient elution (0-1 min, 2%B; 1-2 min, 2%-5%B; 2-4 min, 5%-7%B; 4-6 min, 7%-24%B; 6-10 min, 24%-42%B; 10-12 min, 42%-54%B; 12-15 min, 54%-76%B; 15-18 min, 76%-100%B), the flow rate was set to 0.3 mL·min-1, the column temperature was 30 ℃, the injection volume was 2 µL. The mass spectrographic analysis was used with electrospray ionization (ESI), sample MS data was acquired by time-dependent MSE in negative ion mode, the collection range was m/z 50-1 200 (supernatant) and m/z 50-3 000 (precipitate). Then the chemical constituents were identified by the information of retention time, accurate relative molecular mass and secondary mass spectrum fragment. Result:Totally 61 compounds were identified in the supernatant, including tannins, phenolic acids, flavonoids, amino acids, organic acids, fatty acids, etc. Totally 15 compounds were identified in the precipitate, including tannins, phenolic acids, flavonoids, fatty acids, etc. Conclusion:The hydrolyzed tannin of Sanajon oral liquid may be the potential material basis of its precipitate, and its precipitate is likely to be a complex precipitate mainly composed of ellagic acid and tanned red. The established UPLC-Q-TOF-MSE can quickly and comprehensively analyze the chemical composition of Sanajon oral liquid, which can provide a scientific basis for the researches of its material basis, precipitation mechanism and quality control.
