Therapeutic Effect and Mechanism of Taohong Siwu Granule on Rats with Dysmenorrhea Due to Cold Coagulation and Blood Stasis
10.13422/j.cnki.syfjx.20201404
- VernacularTitle:桃红四物颗粒对寒凝血瘀痛经大鼠的治疗作用及机制
- Author:
Shan WU
1
;
Meng-meng WANG
1
;
Dai-yin PENG
1
;
Wei-dong CHEN
1
;
Zong-hao WU
2
;
Lan HAN
1
Author Information
1. School of Pharmacy of Anhui University of Chinese Medicine, AnHui Province Key Laboratory of Chinese Medicinal Formula, Hefei 230012, China
2. Hefei Huafang Pharmaceutical Science & Technology Co. Ltd., Hefei 230088, China
- Publication Type:Research Article
- Keywords:
Taohong Siwu granule;
cold coagulation and blood stasis;
dysmenorrhea;
rat
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(14):111-116
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effect of Taohong Siwu granule on dysmenorrhea rats. Method:Forty-eight healthy SD female rats were randomly divided into six groups: control group, model group, Taohong Siwu granule groups (18, 9, 4.5 g·kg-1) and Fuke Qianjin tablet group (0.08 g·kg-1). The rats were given Bugaorer (0.35 mg·kg-1) every day and ice-water bath for 8 minutes for 10 consecutive days. Oxytocin was injected intraperitoneally on the 11th day. The writhing reaction of rats was observed, and the effect of Taohong Siwu granules on hemorheology was measured. The levels of 6-ketoprostacyclin F1α(6-keto-PGF1α), thromboxane B2(TXB2), prostaglandinF2α（PGF2α）, PGE2 of rats were detected by enzyme-linked immunosorbent assay (ELISA). Ca2+ in uterine tissues was scanned by laser confocal microscopy. Result:Each dose of Taohong Siwu granule group reduced the number of writhing, the time of writhing and the incidence of writhing in dysmenorrhea rats, which were significantly lower than those in model group (P<0.05，P<0.01). Compared with the model group, high, medium and low-dose Taohong Siwu granules groups could reduce blood viscosity and plasma viscosity, and Taohong Siwu granules can significantly reduce the content of TXB2, Ca2+，PGF2α, and significantly increase the content of 6-keto-PGF1α，PGE2 (P<0.05，P<0.01). Conclusion:Taohong Siwu granule can significantly alleviate dysmenorrhea symptoms in rats. The mechanism may be correlated with the contents of 6-keto-PGF1α, TXB2 and PGF2α, PGE2 and Ca2+.
