Effect of Shaoyaotang on Expressions of TLR4, NF-κB p65 and IL-6 in Rats with Damp-heat Ulcerative Colitis
10.13422/j.cnki.syfjx.20201403
- VernacularTitle:芍药汤对湿热内蕴型溃疡性结肠炎大鼠TLR4，NF-κB p65和IL-6表达的调控作用
- Author:
Min XU
1
;
Feng-yi WANG
1
;
Dang-sheng ZHAO
1
;
Xiao-wei PU
1
;
Lei ZHANG
1
;
Xiao-yuan ZHANG
1
;
Lin LI
1
;
Shu-xia LI
1
;
Zhi-ping LI
1
Author Information
1. Gansu University of Traditional Chinese Medicine, Lanzhou730000, China
- Publication Type:Research Article
- Keywords:
Shaoyaotang;
ulcerative colitis;
Toll-like receptor 4 (TLR4);
nuclear factor-kappaB p65 (NF-κB p65);
interleukin 6(IL-6)
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(14):53-58
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the mechanism of Shaoyaotang in the treatment of ulcerative colitis (UC) based on toll-like receptor 4 (TLR4)/nuclear factor kappaB (NF-κB) signaling pathway. Method:A total of 50 Wistar rats were selected, including half male and half female. The damp-heat UC rat model was replicated by the methods of the combination of diseases and syndromes and the combination of 2, 4, 6-nitrobenzene sulfonic acid (TNBS) and ethanol. After the successful modeling, the model rats were randomly divided into model group, salazulesulfonate group, and low, medium and high-dose Shaoyaotang groups, and 10 rats (half male and half female) were selected as the blank control group. Low, medium and high-dose Shaoyaotang groups were given 6, 12, 24 g·kg-1 by gavage, and salazonyl arsenic group was given 1 g·kg-1 by gavage. Blank control group was given the equal volume of normal saline for 21 consecutive days. Colon samples were collected after the last administration, and the expressions of TLR4, NF-κB p65 and IL-6 mRNA in colon tissues were detected by fluorescent quantitative polymerase chain reaction (Real-time PCR）, and the expressions of TLR4, NF-κB p65 and IL-6 protein in colon tissues were detected by Western blot. Result:Compared with the blank control group, the relative expressions of TLR4, NF-κB p65, IL-6 mRNA and protein in the model group were significantly increased (P<0.05). Compared with the model group, the expression levels of TLR4, NF-κB p65 and IL-6 mRNA and protein in the salazopyridine group and Shaoyaotang groups were significantly decreased (P<0.05). Conclusion:Shaoyaotang can inhibit the development of UC by regulating the expressions of TLR4, NF-κB p65 and IL-6 mRNA and proteins in the TLR4/NF-κB pathway.
