Analysis of Flavonoid Content and Key Gene Expressions in Sprout and Seedling of Tartary Buckwheat
10.13422/j.cnki.syfjx.20201513
- VernacularTitle:苦荞芽和苗中黄酮类化合物含量差异及关键基因表达分析
- Author:
Guang-tao QIAN
1
;
Er-kun CHAO
1
;
Wei SUN
2
;
Wei YANG
2
;
Gang-qiang DONG
3
;
Wei DU
4
;
Qing-fu CHEN
5
;
Zhi-hui ZHU
1
;
Wei SHENG
1
;
Jian-ping XUE
1
Author Information
1. Key Laboratory of Resource Plant Biology of Anhui Province，School of Life Sciences， Huaibei Normal University， Huaibei 235000， China
2. Beijing Key Laboratory for Identification and Safety Evaluation of Chinese Medicine， Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences， Beijing 100700， China
3. Amway （China） Botanical R&D Centre， Wuxi 214115， China
4. Agilent Technology （China） Co. Ltd.， Beijing 100102， China
5. Research Center of Buckwheat Industry Technology， Guizhou Normal University， Guiyang 550001， China
- Publication Type:Research Article
- Keywords:
tartary buckwheat sprout;
tartary buckwheat seedling;
flavonoids;
gene expression;
germination;
dynamic change
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(13):174-180
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To reveal the dynamic changes of flavonoids secondary metabolites and relevant genes expressions in the process of germination of tartary buckwheat seeds by investigating the content of catechins，epicatechins，rutin，and quercetin，and the expressions of their relevant genes in tartary buckwheat sprouts and seedlings，in order to provide scientific basis for the selection of high-quality, high-nutrition tartary buckwheat sprouts.Method:Contents of catechin，epicatechin，rutin，and quercetin in tartary buckwheat sprouts and seedlings were detected by UPLC-ESI-QQQ-MS，and the expression levels of genes relating to flavonoids synthesis in tartary buckwheat sprouts and seedlings were detected by real-time quantitative PCR.Result:There were differences between tartary buckwheat sprouts and seedlings in the relative contents of catechin，epicatechin，rutin and quercetin，as well as the expressions of relevant genes in the synthesis pathway, including FtPAL，FtC4H，Ft4CL，FtCHS，FtCHI，FtF3H，FtF3'H，FtFLS，FtDFR，FtLAR，FtANS，FtANR. The contents of flavonoids and the expressions of relevant genes in tartary buckwheat sprouts were higher than those in tartary buckwheat seedlings.Conclusion:The higher accumulation of secondary metabolites and flavonoids in tartary buckwheat sprouts may be related to tartary buckwheat seeds' resistance to the external environment in the initial growth stage of germination. From the perspective of application，there are more flavonoids in tartary buckwheat sprouts than in tartary buckwheat seedlings, indicating that tartary buckwheat sprouts have a higher nutritional value.
