Terpenoids from Stems and Leaves of Aphanamixis grandifolia
10.13422/j.cnki.syfjx.20201311
- VernacularTitle:大叶山楝枝叶中的萜类成分
- Author:
Yan-wu CHEN
1
;
Fa-wu DONG
1
;
Tian-li QIN
1
;
Fan ZHANG
1
;
Shi-li WU
1
;
Miao-yan YANG
1
;
Ju YANG
1
;
Hong-ping HE
1
Author Information
1. Yunnan Key Laboratory for Dai and Yi Medicine， School of Traditional Chinese Medicine， Yunnan University of Chinese Medicine， Kunming 650500， China
- Publication Type:Research Article
- Keywords:
Meliaceae;
Aphanamixis;
A. grandifolia;
chemical constituents;
terpenoids
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(13):168-173
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Aphanamixis grandifolia，a perennial herb of genus Aphanamixis （Meliaceae），has effects in soothing activating collaterals，dredging paisy，expelling wind-evil and removing wetness. This paper aimed to investigate terpenoids from the stems and leaves of A. grandifolia and reveal the effective substances. Method:Totally 22 kg leaves and twigs of A. grandifolia were extracted with 90% EtOH for three times by heating reflux. These extracts were decompressed and concentrated，and then dissolved in water. The solvent was successively extracted with petroleum ether and ethyl acetate. The chemical constituents from petroleum ether and ethyl acetate fractions were isolated by macroporous，silica gel，Sephadex LH-20 and ODS columns，and their chemical structures were determined through MS，NMR analysis（1H and 13C-NMR）and spectroscopic data from literatures，respectively. Result:Twelve compounds were obtained and identified as 16α-hydroxy-3-oxo-24-methyllanosta-7，9（11），24（31）-triene-21-oic acid （1），23（E）-cycloart-en-25-ethoxy-3-ol（2），23（Z）-9，19-cycloart-ene-3β，25-diol（3），23（E）-cycloart-en-3β，25-diol（4），labda-8，13-（E）-dien-15-ol（5），labda-7，13-（E）-dien-15-ol（6），vulgarol（7），（S，E）-6-[6-（5，5-dimethyl-4-oxo-4，5-dihydrofuran-3-yl]-2-methylhepta-1，6-dien-1-yl]-4-methyl-5，6-dihydro-2H-pyran-one（8），nemoralisin（9），nemoralisin C（10），1S，4R，5S，6R，7S，10S-1（5），6（7）-diepoxy-4-guaiol（11） and 1S，4S，5S，10R-4，10-guaianediol（12），respectively. Conclusion:The structures involves triterpenoids，diterpenoids and sesquiterpennoids，and eight of them （1-3，5-8 and 12） are obtained from A. grandifolia for the first time. Those compounds are also isolated from the geneus Aphanamixis for the first time.
