Efficacy Evaluation of Jinchai Kangbingdu Capsule Based on Coronaryvirus Pneumonia with 'Hanshi Yidu Xifei' syndrome Model
10.13422/j.cnki.syfjx.20201401
- VernacularTitle:基于冠状病毒肺炎寒湿疫毒袭肺证病证结合模型的金柴抗病毒胶囊疗效评价
- Author:
Shan-shan GUO
1
;
Dan LI
2
;
Yu-jing SHI
1
;
Yan-yan BAO
1
;
Rong-hua ZHAO
1
;
Zi-han GENG
1
;
Jing SUN
1
;
Lei BAO
1
;
Ying-jie GAO
1
;
Xiao-lan CUI
1
Author Information
1. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
2. Shineway Pharmaceutical Group Ltd., Shijiazhuang 051400， China
- Publication Type:Research Article
- Keywords:
Jinchai Kangbingdu capsule;
coronavirus;
'Hanshi Yidu Xifei' syndrome;
inflammatory factor;
combination of disease and syndrome
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(13):1-7
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the therapeutic effect of Jinchai Kangbingdu capsule based on human coronavirus pneumonia with 'Hanshi Yidu Xifei' syndrome model, in order to provide experimental basis for evaluating its effect in preventing and treating coronavirus infection. Method:The 48 Balb/c mice were randomly divided into normal group, virus infection group, cold-dampness group, cold-dampness epidemic toxin lung syndrome model group, and high and low-dose Jinchai Kangbingdu capsule groups (1.76, 0.88 g·kg-1·d-1). A cold-dampness stimulation combined with human coronavirus 229E infection was used to imitate human coronavirus pneumonia with 'Hanshi Yidu Xifei' syndrome model. Behavioral characteristics, lung index, viral load, and lung tissue pathological changes in Balb/c mice were observed to evaluate the therapeutic effect of Jinchai Kangbingdu capsules. The contents of interleukin-6（IL-6），IL-10，tumor necrosis factor-α（TNF-α），interferon-γ（IFN-γ） in lung tissue and motilin（MTL），gastrin（GAS） in serum were detected by enzyme-linked immunosorbent assay(ELISA), and the contents of CD4+ T cells, CD8 + T cells, and B cells in peripheral blood were detected by flow cytometry. Result:Compared with the cold-dampness epidemic toxin lung syndrome model group, Jinchai Kangbingdu capsule can increase the activity and response ability of 'Hanshi Yidu Xifei' syndrome model mice, and change the skin and stool status of mice. High and low-dose Jinchai Kangbingdu capsule groups can significantly reduce the lung index (P<0.01), while significantly increased the content of CD4+ T cells, CD8+ T cells, and B cells (P<0.05, P<0.01). Low-dose Jinchai Kangbingdu capsule group could significantly decrease the MTL content in serum and the levels of IL-6, IL-10, TNF-α, IFN-γ in lung tissue (P<0.01), whereas alleviate the pathological damage of lung tissue. Conclusion:Jinchai Kangbingdu capsule showed a therapeutic effect on human coronavirus pneumonia with 'Hanshi Yidu Xifei' syndrome model, and can improve the behavioral characterization and gastrointestinal index level of cold-dampness syndrome, while reduce lung index and viral load in lung tissue. The mechanism may be related to the decrease of the content of inflammatory factors and the increase of the number of lymphocytes.
