Nucleosides from Acorus tatarinowii and Their Transformation Pathway
10.13422/j.cnki.syfjx.20201411
- VernacularTitle:石菖蒲中核苷类成分的分离鉴定及转化途径分析
- Author:
Sen JIAO
1
;
Shu-jie MAO
2
;
Yao-hua LIANG
2
;
Liang-mian CHEN
2
;
Li-hua YAN
2
;
Zhi-min WANG
2
Author Information
1. Henan University of Chinese Medicine， Zhengzhou 450000， China
2. National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines， Institute of Chinese Materia Medica， China Academy of Chinese Medical Sciences，Beijing 100700，China
- Publication Type:Research Article
- Keywords:
Acorus tatarinowii;
water extract;
phenylpropanoids;
nucleosides;
nucleotides;
transformation pathway
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(12):145-153
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the water soluble chemical constituents in rhizoma of Acorus tatarinowii and transformation pathway of nucleosides in the process of water extraction. Method:Compounds were isolated and purified by column chromatography on macroporous resin，Sephadex LH-20，ODS and preparative HPLC. Their structures were identified on the basis of physicochemical properties and spectral data. Nucleosides were identified from aqueous extract of A. tatarinowii，and their stability was investigated by HPLC. The possible transformation pathways of nucleosides in aqueous extract of A. tatarinowii were studied by nucleotide addition test. Result:Eleven compounds including four nucleosides，four phenylpropanoids，two alkaloids and a furfural were isolated，and identified as uridine（1），adenine（2），guanosine（3），adenosine（4），5-hydroxymethylfurfural（5），5-（hydroxymethyl）-1H-pyrrole-2-carboxaldehyde（6），（threo）1'，2'-dihydroxyasarone（7），（erythro）1'，2'-dihydroxyasarone（8），acoraminol A（9），acoraminol B（10），and tatarine A（11）. The chromatographic peaks of compounds 1-4 and cytidine were identified from aqueous extract of A. tatarinowii by HPLC. After ultrasonic extraction for 0.5 h，the stability of nucleosides in water was poor. After ultrasonic extraction for 3 h or refluxing extraction for 0.5 h，the stability of nucleosides in water was good. Four transformation pathways including 5'-cytidylic acid→cytidine，uridine monophosphate→uridine，guanosine monophosphate，guanosine and adenosine-5'-monophosphate，adenosine 5'-diphosphate，adenosine 5'-triphosphorate，adenosine，adenine might exist in water extract of A. tatarinowii. Conclusion:Compounds 1-4 and 6 were isolated from the genus Acorus for the first time. These compounds further enriched the chemical constituents of A. tatarinowii. The stability and transformation pathway of nucleosides in A. tatarinowii provides reference data for the analysis of nucleosides in A. tatarinowii and other traditional Chinese medicine.
