Effect of Decomposed Zuoguiwan Recipes in Improving BMD of Ovariectomized Rats by Regulating OPG/RANKL Pathway Mediated by β2AR
10.13422/j.cnki.syfjx.20201224
- VernacularTitle:左归丸拆方通过调节β2-AR介导的OPG/RANKL信号通路提高去卵巢大鼠骨密度
- Author:
Ming-yue ZHANG
1
;
Feng TAN
1
;
Su-min YE
1
;
Yi CHAI
1
;
Fei-xiang LIU
1
;
Cong-ying MAI
1
;
Xing LI
1
;
Dao-ming XU
2
;
Qiao-ling FAN
1
Author Information
1. School of Basic Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China
2. Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, China
- Publication Type:Research Article
- Keywords:
Zuoguiwan;
decomposed Zuoguiwan recipes;
osteoprotegerin;
seeking Yin in Yang;
receptor activator of nuclear factor kappa-B ligand (RANKL);
osteoprotegerin (OPG);
β2-adrenergic receptor (β2AR)
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(12):49-55
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the mechanism of decomposed Zuoguiwan（ZGW） recipes in treating ovariectomized osteoporosis rats. Method:Forty Sprague-Dawley female rats were equally and randomly divided into Sham-operated group, ovariectomized model group, positive group, and low and high-dose ZGW groups. After 12 weeks of administration by gavage, the bone mineral density (BMD) of rats' distal femur was measured by micro-CT, the morphology of bone tissue were observed by hematoxylin-eosin staining (HE), β-cross-linked c-telopeptide of type Ι collagen (β-CTX) and bone-specific alkaline phosphatase (BALP) in serum were detected by enzyme-linked immunosorbent assay (ELISA), and the mRNA and protein expressions of β2AR, OPG and RANKL were evaluated by Western blot analysis and real-time quantitative polymerase chain reaction （PCR）. Result:Compared with Sham-operated group, BMD of rats in ovariectomized model group was decreased (P<0.01), morphology of bone tissue was destroyed, serum BALP was reduced, while β-CTX was boosted (P<0.01)，mRNA and protein expressions of OPG in tibia were reduced, while RANKL were increased, and mRNA and protein expressions of β2AR in the hypothalamus were decreased (P<0.05, P<0.01). Compared with ovariectomized model group, BMDs of rats in low and high-dose ZGW groups were increased (P<0.01), morphology of bone tissue was repaired, serum BALP and mRNA and protein expressions of OPG in tibia were up-regulated (P<0.05, P<0.01), whereas serum β-CTX and mRNA and protein expressions of β2AR in the hypothalamus and RANKL in tibia were down-regulated (P<0.05, P<0.01). Conclusion:Yang-nourishing components in decomposed Zuoguiwan recipes can improve BMD of ovariectomized rats by regulating OPG/RANKL pathway mediated by β2AR. "Seeking Yin in Yang" is a crucial mechanism of Zuoguiwan in treating ovariectomized osteoporosis in rats.
