Cloning and Bioinformatic Analysis of ARPI Gene from Glycyrrhiza glabra
10.13422/j.cnki.syfjx.20191951
- VernacularTitle:光果甘草ARPI基因的克隆和生物信息学分析
- Author:
Shao-kai TIAN
1
;
Jia-ming HOU
1
;
Zhi-qiang GAO
1
;
Lin YANG
1
;
Zhi-xin ZHANG
1
;
Ying LIU
1
Author Information
1. School of Life Sciences, Beijing University of Chinese Medicine, Beijing 102488, China
- Publication Type:Research Article
- Keywords:
Glycyrrhiza glabra;
auxin responsive protein;
auxin/indole acetic acid protein;
glycyrrhizic acid;
bioinformatics;
open reading frame;
GgARPI gene
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(4):185-190
- CountryChina
- Language:Chinese
-
Abstract:
Objective::To clone the complementary deoxyribonucleic acid (cDNA) of auxin/indole acetic acid protein (Aux/IAA) from Glycyrrhiza glabra (GgARPI) and analyze its sequence by bioinformatics. Method::RNA was extracted from fresh root of G. glabra, the cDNA sequence of GgARPI gene was cloned by reverse transcription polymerase chain reaction (RT-PCR), then sequencing and bioinformatic analysis were performed. Result::The GgARPI cDNA sequence with the full length of 686 bp was obtained from G. glabra. The full open reading frame (ORF) was 585 bp, encoding 194 amino acid residues. The bioinformatic analysis showed that the protein coded by GgARPI was a stable hydrophilic protein, with a relative molecular weight of 21.95 kDa and isoelectric point of 6.85.It contained no signal peptides or transmembrane domain. Its secondary structure mainly consisted of random coil. An Aux/IAA superfamily was included in the conversed domain. Homology analysis indicated that it had a close evolutionary relationship with leguminous plants, and a distant evolutionary relationship with monocotyledon, such as Setaria italica. Conclusion::GgARPI cDNA sequence is successfully cloned from G. glabra for the first time, which will lay a foundation for studying the function of GgARPI and explaining the molecular regulatory mechanism of biosynthesis of glycyrrhizic acid in G. glabra.
