Structures Characteristics and Bioactivity of Polysaccharide CALB-2 from Aurantii Fructus
10.13422/j.cnki.syfjx.20201714
- VernacularTitle:枳壳均一多糖CALB-2结构表征及生物活性
- Author:
Wu-jing WANG
1
;
Yan-ni YANG
2
;
Zi-he DING
2
;
Ren-xing ZHONG
2
;
Tian-yi XIA
2
;
Shu-yuan LI
2
;
Yi WANG
2
;
Sheng-qing LI
1
;
Zun-peng SHU
2
Author Information
1. School of Pharmaceutical Sciences，Guangzhou University of Chinese Medicine， Guangzhou 510006，China
2. Guangdong Standardized Processing Engineering Technology Research Center of Traditional Chinese Medicine（TCM），School of TCM， Guangdong Pharmaceutical University，Guangzhou 510006，China
- Publication Type:Research Article
- Keywords:
Aurantii Fructus;
polysaccharides CALB-2;
separation and purification;
structure characterization;
antimyocardial injury
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(16):154-160
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To isolate and purify a polysaccharide CALB-2 fraction from Aurantii Fructus，and analyze its basic chemical structure, morphological characteristics and bioactivity. Method:A refined CALB-2 was obtained from Aurantii Fructus by hot water extraction，then separated and purified by ion exchange resin，ion exchange agarose gel and propylene dextran gel to obtain homogeneous polysaccharide CALB-2. The molecular mass of CALB-2 was determined by high performance liquid chromatography （HPLC）. Monosaccharide composition analysis of CALB-2 was conducted by methylation analysis and Smith degradation. Structural analysis and morphological characterization were conducted by infrared scanning （IR） and scanning electron microscopy （SEM） analysis. Antioxidant activity of CALB-2 was studied by using H2O2-induced cardiomyocyte oxidative damage model. Result:CALB-2 was a homogeneous polysaccharide and the molecular weight of CALB-2 was estimated to be 3.57×107 Da，which was proved to be a kind of highly branched acidic polysaccharides in IR analysis, methylation analysis and Smith degradation, mainly present in form of 1→3，4 bonds. Through SEM observations，we indicated that the molecular morphology of CALB-2 was amorphous solid. The in vitro activity test showed that CALB-2 had obvious protective effects on injury of H9c2 myocardial cells induced by H2O2. Conclusion:CALB-2 is a kind of homogeneous polysaccharide extracted from Aurantii Fructus， with an anti-cardiomyocyte oxidative damage effect, laying a theoretical foundation for further study of Aurantii Fructus polysaccharides．
