Analysis of Influence of Jiangxi Characteristic Processing Technology on Chemical Compositions of Aurantii Fructus Based on UPLC-Q/TOF-MS
10.13422/j.cnki.syfjx.20201069
- VernacularTitle:基于UPLC-Q/TOF-MS分析江西特色炮制工艺对枳壳化学成分的影响
- Author:
Jing ZHU
1
;
En YUAN
1
;
Jun-yong MOU
1
;
Ling-yun ZHONG
1
;
Jin-lian ZHANG
1
;
Ming YANG
1
;
Hai-ping LIU
2
Author Information
1. Jiangxi University of Traditional Chinese Medicine,Nanchang 330004,China
2. Guilin Medical University,Guilin 541004,China
- Publication Type:Research Article
- Keywords:
Aurantii Fructus;
processing;
decoction pieces;
chemical constituents;
ultra-high performance liquid chromatography-quadrupole-time of flight mass spectrometry (UPLC-Q/TOF-MS);
principal component analysis (PCA);
partial least squares-discriminant analysis (PLS-DA)
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(16):142-153
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To compare the effects of different processing techniques on the chemical constituents of Aurantii Fructus for screening the dominant decoction pieces. Method:UPLC-Q/TOF-MS was used to detect the chemical constituents of Aurantii Fructus, chromatography separation was achieved on an ACQUITY UPLC BEH C18 column (2.1 mm×100 mm, 1.7 μm), and gradient elution was performed with 0.1% formic acid aqueous solution (A)-0.1% formic acid acetonitrile solution (B) as mobile phase (0-10 min, 5%-35%B; 10-18 min, 35%-75%B; 18-21 min, 75%-100%B; 21-24 min, 100%B; 24-24.1 min, 100%-5%B; 24.1-28 min, 5%B). Data acquisition was carried out in electrospray ionization (ESI) under the positive ion mode, the scanning range was m/z 50-1 200. The chemical constituents in methanol extract of Aurantii Fructus were identified according to reference substance, relative molecular weight, mass spectrometric cleavage rule and literature information. SIMCA-P 13.0 software was used to establish principal component analysis (PCA) model and partial least squares-discriminant analysis (PLS-DA) model of Aurantii Fructus processed products, PCA score plot, PLS-DA loading plot and variable importance in the protection (VIP) values were obtained to screen the material basis for the main differences before and after processing of Aurantii Fructus. Result:A total of 54 chemical components were identified by UPLC-Q/TOF-MS. PCA indicated that there were significant differences among different groups of Aurantii Fructus processed by different methods. A total of 14 chemical components with VIP value >1 were screened by PLS-DA as the main chemical markers for the differences before and after processing, including hesperidin, poncirin, narirutin, etc. The comprehensive weighted score showed that the content of effective components in Aurantii Fructus processed with honey bran was the highest. Conclusion:The contents of chemical constituents in Aurantii Fructus before and after processing are significantly changed. Flavonoids are the most important compounds to distinguish different processed products of Aurantii Fructus. Aurantii Fructus processed with honey bran is the dominant variety.
