Chemical Constituents of Corydalis impatiens and Their Inhibitory Effect on Proliferation of Hepatoma Cells
10.13422/j.cnki.syfjx.20201711
- VernacularTitle:赛北紫堇化学成分及其对肝癌细胞的增殖抑制作用
- Author:
Ze-dong NAN
1
;
Guang-tian HAN
2
;
Xi-an LI
2
;
Hua-zhong REN
2
;
Jiang YU
2
;
Shou-feng WANG
3
;
Li GUO
1
Author Information
1. Chengdu University of Traditional Chinese Medicine（TCM），School of Pharmacy，Southwest Genuine Medicinal Materials Collaborative Innovation Center，State Key Laboratory of Resources Systems Research and Development Utilization of TCM，Chengdu 611137，China
2. Leshan Vocational & Technical college，Leshan 614000，China
3. Sichuan University of Science and Engineering，College of Chemical Engineering，Zigong 643000，China
- Publication Type:Research Article
- Keywords:
Corydalis impatiens;
chemical constituents;
structure elucidation;
cytotoxic activity
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(15):163-168
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To systematically investigate the chemical constituents of 90% ethanol extract of Corydalis impatiens，and evaluate their inhibitory effect on cell proliferation in vitro. Method:The chemical constituents were isolated and purified by silica gel，Sephadex LH-20，ODS column chromatography and semi-preparative high performance liquid chromatography （HPLC）.the structures were identified by spectroscopy methods such as NMR and MS，as well as analysis of physicochemical properties and/or comparison with literature data，and the inhibitory activities of 13 compounds on HepG2 and SMMC-7721 cells were measured by methyl thiazolyl tetrazolium （MTT） method. Result:Thirteen known compounds were isolated and identified from 90% ethanol extract of Corydalis impatiens as ethyl-5-hydroxy-2-pyridinecarboxylate（1），coryhumolide（2），3，4-cis-3，4-dihydroxy-β-ionone（3），megastigmane（4），9-hydroxy-4，7-megastigmadien-3-one（5），blumenol A（6），indole-3-carboxy acid（7），1-methyl-[1，2，4]triazolo[4，3-b][1，2，4]triazin-7-one （8），adenine（9），nicotinamide（10），2-hydroxymethyl-5-pyridinol（11），adenosine（12）， and β-daucosterol（13）. Cytotoxicities assay showed that the IC50 value of compound 3 for the hepatic cell line HepG2 was 24.7 μmol·L-1 （positive control drug cisplatin：4.8 μmol·L-1），and IC50 value of compound 4 for the hepatic cell line SMMC-7721 was 13.8 μmol·L-1（positive control drug cisplatin：5.4 μmol·L-1）. Conclusion:Compound 1 was a new natural compound，compounds 3-8 were obtained from genus Corydalis for the first time，and compounds 2，9-12 were isolated from this plant for the first time. Compound 3 exhibited weak inhibitory effect on hepatic cell line HepG2，and compound 4 exhibited moderate inhibitory effect on hepatic cell line SMMC-7721.The other compounds rest of ones exhibited no obvious inhibitory effect on hepatic cell line HepG2 or SMMC-7721.
