Simultaneous Determination of 7 Phenolic Acids in Lycopus lucidus var. hirtus Rhizome by HPLC-PDA
10.13422/j.cnki.syfjx.20201615
- VernacularTitle:HPLC-PDA同时测定地笋中7种酚酸的含量
- Author:
Xiao-lan HUANG
1
;
Xu-feng HE
2
;
Qin YANG
3
;
Dong-qin GUO
1
;
Qian WANG
1
;
Nong ZHOU
1
Author Information
1. College of Biological and Food Engineering，The Chongqing Engineering Laboratory for Green Cultivation and Deep Processing of The Three Gorges Reservoir Area's Medicinal Herbs，Chongqing Three Gorges University，Chongqing 404000，China
2. Chongqing Wanzhou Food and Drug Inspection Institute，Chongqing 404000，China
3. College of Traditional Chinese Medicine，Chongqing Three Gorges Medical College，Chongqing 404120，China
- Publication Type:Research Article
- Keywords:
Lycopus lucidus var. hirtus rhizome;
phenolic acids;
high performance liquid chromatography-photo-diode array（HPLC-PDA）;
rosemary acid;
principal component analysis;
cluster analysis
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(15):156-162
- CountryChina
- Language:Chinese
-
Abstract:
Objective:A high performance liquid chromatography-photo-diode array（HPLC-PDA） method for the simultaneous determination of the 7 phenolic acids including danshensu，protocatechuic acid，protocatechuic aldehyde，chlorogenic acid，caffeic acid，ferulic acid and rosemary acid in Lycopus lucidus var.hirtus rhizome，analyzing and evaluating the phenolic acids in L.lucidus var.hirtus rhizome collected from different habitats，is reported here. Method:The sample was extracted by ultrasonic with 80% methanol solution，7 kinds of phenolic acids were separated on a CAPCELL PAK C18 column （4.6 mm×250 mm，5 μm） with a mobile phase consisting of methanol-0.02% formic acid aqueous （pH 3.10）by gradient elution，The detection wavelength was at 279，324 nm， the column temperature was 30 ℃ with 20 μL injection volume and the flow rate was 1.0 mL·min-1. Result:The 7 Phenolic acids had a good linear relationship （r≥0.999 9） within their respective mass concentration ranges，the average recovery was 96.49%-103.45% and the RSD was 0.5%-2.8%，the limit of determination was 0.008-0.046 mg·L-1 and the limit of quantification was 0.027-0.154 mg·L-1.The 7 kinds of phenolic acids were all detected in L.lucidus var.hirtus rhizome and the total amount was between 5 811.01 and 11 747.23 µg·g-1 ， the average amount was 7 421.05 µg·g-1.The content of 7 phenolic acids was different and the rosemary acid was the highest in all the samples with an average of 7 111.19 µg·g-1 the ratio to the total phenolic acids was 95.8%.The results of principal component analysis and cluster analysis showed that the quality of L.lucidus var.hirtus rhizome from Heze city in Shandong province was better，followed by Wanzhou district in Chongqing. Conclusion:The method was simple，sensitive，accurate，practical and reliable，and is suitable for the content determination of phenolic acid in L. lucidus var. hirtus rhizome.It is expected to provide a reference for the improvement of quality standard and a new idea for the development and utilization of L.lucidus var.hirtus rhizome.
