ISSR Analysis of Genetic Relationship and Population Structure of Turpinia arguta Fresh Leaves in Jiangxi Province
10.13422/j.cnki.syfjx.20201046
- VernacularTitle:江西省锐尖山香圆亲缘关系与群体结构的ISSR分析
- Author:
Xiao-yun WANG
1
;
Lian ZHANG
1
;
Lan CAO
1
;
Fang LIANG
2
Author Information
1. Jiangxi University of Traditional Chinese Medicine，Nanchang 330004，China
2. Yuzhang Normal University，Nanchang 330004，China
- Publication Type:Research Article
- Keywords:
Turpinia arguta;
inter-simple sequence repeat （ISSR）;
molecular marker;
genetic relationship;
population genetic structure;
polyacrylamide gel electrophoresis （PAGE）;
unweighted pair group method with arithmetic mean （UPGMA）
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(15):150-155
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore genetic relationship and population structure of Turpinia arguta in six locations of Jiangxi province by inter-simple sequence repeat （ISSR） molecular marker technique， and to provide theoretical basis for the protection and utilization of this medicinal material resource. Method:A total of 22 samples from six locations in four counties in Jiangxi province were collected， and genomic DNA was extracted by kit method. Polymerase chain reaction （PCR） amplification was performed using sixty-four universal ISSR molecular marker primers， and the products were detected with polyacrylamide gel electrophoresis （PAGE）. NTsys 2.10e software was selected to calculate the genetic similarity coefficient by unweighted pair group method with arithmetic mean （UPGMA） and cluster analysis. Population genetic structure was analyzed by Structure 2.1 software. Result:A total of forty-eight ISSR primers were amplified to obtain the product， the percent of polymorphic bands ranged from 45.45% to 100%. UPGMA cluster analysis showed that these plant individuals could not be clustered according to their respective executive locations. Analysis of population genetic structure showed that 22 samples of T. arguta could be divided into three populations. Conclusion:There is gene exchange among the populations of T. arguta in Jiangxi province， and it can affect the genetic structure of germplasm resources from different geographical sources.
