Identification of Decoction Pieces and Standard Decoction of Ophiopogonis Radix and Liriopes Radix by TLC
10.13422/j.cnki.syfjx.20201048
- VernacularTitle:麦冬与山麦冬饮片及标准汤剂的薄层色谱鉴别
- Author:
Yu HAO
1
;
Qi-shu JIAO
1
;
Yan-yan ZHOU
1
;
Ru-na JIN
1
;
Chun-miao XUE
2
;
Shou-gang SHI
3
;
Zheng-jun HUANG
3
;
Yun-tao DAI
1
Author Information
1. Institute of Chinese Materia Medica， China Academy of Chinese Medical Sciences， Beijng 100700，China
2. Dongzhimen Hospital， Beijing University of Chinese Medicine， Beijing 100700，China
3. Sunflower Pharmaceutical Group （Xiangyang） Longzhong Co.Ltd.，Xiangyang 441003，China
- Publication Type:Research Article
- Keywords:
Ophiopogonis Radix;
Liriopes Radix;
standard decoction;
decoction pieces;
quality control;
compound preparations;
thin layer chromatography （TLC）
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(15):124-129
- CountryChina
- Language:Chinese
-
Abstract:
Objective:There were 92 kinds of compound preparations containing Ophiopogonis Radix in the 2015 edition of Chinese Pharmacopoeia， but there was no effective method to identify these compound preparations. Because Ophiopogonis Radix and Liriopes Radix are similar in appearance， it is easy to be confused in application. The aim of this study was to set up a thin layer chromatography （TLC） to identify compound preparations containing Ophiopogonis Radix and distinguish Ophiopogonis Radix and Liriopes Radix in the forms of decoction pieces and standard decoction. Method:In this study， decoction pieces of Ophiopogonis Radix and Liriopes Radix were collected and separately prepared as standard decoction. TLC was used to qualitatively identify decoction pieces and standard decoction of Ophiopogonis Radix and Liriopes Radix， and compound preparations containing Ophiopogonis Radix. In the TLC， the lower solution of chloroform-methanol-water （65∶35∶10） was selected as the developing agent and 10% sulfuric acid ethanol solution as the chromogenic agent. Result:The resolution of this TLC was good. Decoction pieces， standard decoction and preparations of Ophiopogonis Radix had the same characteristic strips， which were two bright white fluorescent strips under ultraviolet lamp （365 nm）. But these two characteristic strips were not existed in the TLC of decoction pieces and standard decoction of Liriopes Radix. The corresponding components of both of these two strips were identified as mixture containing saponins by LC-MSn， including ophiopogonin Ra， Tb， ophiopogonin D'， borneol glycoside， ophiopogonin C and Liriope muscari baily saponins C. Conclusion:The established TLC method， which has significant advantages such as high specificity and sensitivity， can be applied to the characteristic identification of decoction pieces and standard decoction of Ophiopogonis Radix， the identification of compound preparations containing Ophiopogonis Radix， and the distinction of Ophiopogonis Radix and Liriopes Radix， thus serving as an effective method to qualitatively identify Ophiopogonis Radix and its compound preparations.
