Protective Effect of Artemisinin on Mice with Systemic Inflammatory Response Syndrome
10.13422/j.cnki.syfjx.20201409
- VernacularTitle:青蒿素对全身炎症反应综合征小鼠的保护作用
- Author:
Hong ZHANG
1
;
Qing YANG
1
;
Li-dong SUN
1
;
Wei-yan CAI
1
;
Yu-jie LI
1
;
Zi-yu YANG
1
;
Qing-sen RAN
1
;
Li LIU
1
;
Xiao-gang WENG
1
;
Qi LI
1
;
Ya-jie WANG
1
;
Xiao-xin ZHU
1
;
Ying CHEN
1
Author Information
1. Institute of Chinese Materia Medica China Academy of Chinese Medical Sciences， Beijing 100700，China
- Publication Type:Research Article
- Keywords:
artemisinin;
systemic inflammatory response syndrome;
inflammatory factor
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(15):20-25
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the protective effect and mechanism of artemisinin on systemic inflammatory response syndrome （SIRS）mice using endotoxin （LPS）-induced SIRS mouse model. Method:Male BALB/c mice aged 5-7 weeks were randomly divided into normal group， LPS model group， low， medium and high-dose artemisinin groups （25， 50， 100 mg·kg-1） and ibuprofen group （39 mg·kg-1）. LPS （10 mg·kg-1） was intraperitoneally injected at the 7th day after the prophylaxis. According to the SIRS clinical diagnostic criteria， the respiratory rate， rectal temperature， lung index， spleen index， glycolipid metabolism， brain tissue inflammatory factors， and phosphorylation of lung tissue inflammation-related proteins were measured. Result:Intraperitoneal injection of LPS significantly reduced the respiratory rate of mice （P<0.05）， body temperature decreased significantly （P<0.01）， spleen index increased significantly （P<0.01）， peripheral blood neutrophil percentage increased significantly （P<0.05）， percentage of monocytes decreased significantly （P<0.01）， thrombocyte decreased （P<0.01）， platelet specific ratio decreased （P<0.01）， total cholesterol content in plasma decreased （P<0.01）， plasma glucose content decreased （P<0.01）. The expression of interleukin-1β increased in hippocampus and cortex of brain tissue （P<0.01）， and the secretion of tumor necrosis factor-α increased in hippocampus and cortex of brain tissue （P<0.01）. The expression of phosphorylated protein STAT1 was increased （P<0.01）， and the expression of phosphorylated protein c-Jun was increased （P<0.01）. After the administration of artemisinin， the body temperature and the respiratory rate of mice induced by LPS were significantly increased， the pathological changes of various organs induced by LPS were alleviated， the hypoglycemia induced by LPS was significantly increased （P<0.05）， the levels of inflammatory factors in hippocampus and cortex was significantly reduced， and the expressions of phosphorylated proteins STAT1 and c-Jun in lung tissue were significantly reduced （P<0.05， P<0.01）. Conclusion:Artemisinin has a significantly protective effect on SIRS mice induced by intraperitoneal injection of LPS possibly by reducing the secretion of inflammatory factors TNF-α and IL-1β.
