Molecular Cloning and Sequence Analysis of GgUGE Gene in Glycyrrhiza glabra
10.13422/j.cnki.syfjx.20200214
- VernacularTitle:光果甘草GgUGE基因的克隆和序列分析
- Author:
Jia-ming HOU
1
;
Shao-kai TIAN
1
;
Lin YANG
1
;
Zhi-xin ZHANG
1
;
Zhi-qiang GAO
1
;
Han-qing WANG
2
;
Ying LIU
1
Author Information
1. School of Life Sciences, Beijing University of Chinese Medicine, Beijing 102488, China
2. School of Pharmacy, Ningxia Medical University, Yinchuan 750004, China
- Publication Type:Research Article
- Keywords:
Glycyrrhiza glabra;
UDP-glucose 4-epimerase;
glycyrrhizic acid;
bioinformatic analysis
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(6):163-167
- CountryChina
- Language:Chinese
-
Abstract:
Objective::To clone the cDNA sequence of UDP-glucose 4-epimerase (UGE) in Glycyrrhiza glabra and analyze its sequence, so as to explore the potential relationship between the UGE gene and the molecular regulatory mechanisms of glycyrrhizic acid biosynthesis. Method::The cDNA sequence of UGE was cloned from the root of G. glabra by reverse transcription polymerase chain reaction (RT-PCR), then sequenced and analyzed by bioinformatics software. Results::A GgUGE cDNA sequence with the full length of 1 121 bp was obtained. The open reading fame (ORF) of GgUGE was 1 053 bp, encoding 350 amino acid residues. The GgUGE cDNA sequence was submitted to GenBank, and the accession No. was MK638908. Sequence analysis showed that GgUGE was an unstable hydrophilic protein, its average relative molecular weight was 39.02 kDa, and isoelectric point was 6.13. It contained no signal peptides or transmembrane domains. Its secondary structure mainly constituted of α-helix and had a conversed domain of UDP-glucose 4-epimerase superfamily. The homologoue analysis showed that the cDNA and amino acid sequences of GgUGE had the closest evolutionary relationship to Leguminosae and relatively distant evolutionary relationship to Salicaceae. Conclusion::In this study, GgUGE cDNA sequence is successfully cloned from G. glabra for the very first time, which will provide reference for studying the function of GgUGE and explaining the molecular regulatory mechanisms of glycyrrhizic acid biosynthesis in G. glabra.
