Analysis on Metabolites of Major Components of Shaoyao Gancaotang in Urine and Feces of Normal and Polycystic Ovary Syndrome Rats
10.13422/j.cnki.syfjx.20192156
- VernacularTitle:芍药甘草汤主要成分在正常及多囊卵巢综合征大鼠尿液和粪便中的代谢产物分析
- Author:
Yi-ting GUO
1
;
Yao CHENG
1
;
Yun-yun SHAO
2
;
Jun-jin LIU
2
;
Xiao-juan FENG
1
;
Jing-ping ZHANG
2
;
Rui-gang HOU
2
Author Information
1. Shanxi Medical University, Taiyuan 030000, China
2. Second Hospital of Shanxi Medical University, Taiyuan 030001, China
- Publication Type:Research Article
- Keywords:
polycystic ovary syndrome;
Shaoyao Gancaotang;
metabolites;
metabolic pathway;
urine;
feces;
intestinal flora
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(6):103-112
- CountryChina
- Language:Chinese
-
Abstract:
Objective::To analyze and identify the metabolites of major components of Shaoyao Gancaotang in urine and feces of normal and polycystic ovary syndrome (PCOS) rats, and to explore the effect of PCOS on the metabolism of Shaoyao Gancaotang. Method::Female SD rats were randomly divided into normal group and PCOS group. PCOS rat model was prepared by administration of letrozole solution for 21 days, and the estrous cycle of rats was observed. UHPLC-QTRAP-MS/MS technique and LightSight 2.3 software were used to analyze and identify the metabolites of major components of Shaoyao Gancaotang in urine and feces of normal and PCOS rats. The mobile phase was water-methanol for gradient elution, the flow rate was 0.3 mL·min-1, electrospray ion source was employed under negative ion mode. Result::Phase Ⅰ and phase Ⅱ reactions mainly occurred in drug metabolism. A total of 27 metabolites were detected in urine of normal rats, and 34 metabolites were detected in urine of PCOS rats. A total of 29 metabolites were detected in feces of normal rats, and 27 metabolites were detected in feces of PCOS rats. Conclusion::The metabolites in the urine of PCOS rats are more diverse than those of normal rats, and the disease status of PCOS may affect the in vivo metabolic pathway of active ingredients in Shaoyao Gancaotang.
