Effect of Bletillae Rhizoma Polysaccharide on PI3K/Akt in Gastric Tissue of GU Model Rats
10.13422/j.cnki.syfjx.20200304
- VernacularTitle:白及多糖对GU模型大鼠胃组织PI3K/Akt的影响
- Author:
Zi-han GONG
1
;
Yan-wei WANG
2
;
Yong-qiang DUAN
1
;
Qiang WANG
1
;
Xiao-yan FU
1
;
Min BAI
1
;
Jun MA
1
;
Yan-li BAI
1
;
Xiao-li SI
1
Author Information
1. Gansu University of Chinese Medicine, Lanzhou 730000, China
2. Beijing Longfu Hospital, Beijing 100010, China
- Publication Type:Research Article
- Keywords:
Bletillae Rhizoma polysaccharide;
gastric ulcer;
phosphatidylinositol 3-kinase (PI3K);
protein kinase B (Akt);
interleukin-2 receptor (IL-2R);
interleukin-4 (IL-4)
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(6):52-57
- CountryChina
- Language:Chinese
-
Abstract:
Objective::To investigate the effect of Bletillae Rhizoma polysaccharide on the expressions of phosphatidylinositol 3-kinase (PI3K), protein kinase B (Akt) gene protein and its mediated cytokines interleukin-2 receptor (IL-2R) and interleukin-4 (IL-4) in gastric tissue of rats with gastric ulcer (GU). Method::Sixty SPF Wistar rats were randomly divided into blank group and model group.The GU model was replicated by direct acetic acid cauterization in model group.The GU model rats were randomly divided into five groups: model group, positive control group, and large, medium and small-dose Bletillae Rhizoma polysaccharide groups, with 10 rats in each group.Rats in blank group and GU model group were given 10 mL·kg-1·d-1 distilled water by gavage, rats in large, medium and small-dose groups were given 0.5, 0.25, 0.125 g·kg-1·d-1 Bletillae Rhizoma polysaccharide by gavage, while rats in positive control group were given 0.3 g·kg-1·d-1 ranitidine by gavage for 15 days.Serum nitric oxide (NO) content, pepsinase activity and cytokines IL-2R and IL-4 levels in rats of each group were measured by enzyme-linked immunosorbent assay (ELISA), PI3K and Akt mRNA expressions were detected by Real-time fluorescent quantitative polymerase chain reaction (Real-time PCR), and PI3K and Akt protein expressions were detected by Western blot. Result::Compared with the blank group, the contents and gene expressions of cytokines IL-2R and IL-4 in gastric tissue were significantly increased, and the PI3K and Akt genes and protein expressions were significantly increased, with statistical significance (P<0.01). Compared with GU model group, the content and gene expressions of IL-2R and IL-4 in large, medium and small-dose Bletillae Rhizoma polysaccharide groups were decreased significantly, and the PI3K and Akt gene and protein expressions were decreased significantly in large-dose Bletillae Rhizoma polysaccharide group, while those in large and medium-dose Bletillae Rhizoma polysaccharide groups were decreased significantly (P<0.05, P<0.01). Conclusion::Bletillae Rhizoma polysaccharide can protect gastric mucosa by down-regulating PI3K and Akt gene and protein expressions and inhibiting abnormal secretion of cytokines IL-2R and IL-4.
