Effects of c -Fos Immunoreactivity on Central Nervous System after Administration of Aspirin.
10.11637/kjpa.2001.14.1.17
- Author:
Chang Mok SON
1
;
Chul Hong KIM
;
Ki Soo YOO
Author Information
1. Department of Anatomy, College of Medicine, Dong -A University, Korea.
- Publication Type:Original Article
- Keywords:
Aspirin;
Pain;
Formalin;
c -Fos;
Neuron;
Rat brain
- MeSH:
Animals;
Aspirin*;
Brain;
Central Nervous System*;
Formaldehyde;
Gyrus Cinguli;
Hippocampus;
Humans;
Male;
Neurons;
Nociceptive Pain;
Rats
- From:Korean Journal of Physical Anthropology
2001;14(1):17-27
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Aspirin is one of the popular non -steroid anti -inflammatory drugs used in the management of pain. This study was performed to investigate the effects of aspirin on c -Fos expression in rat CNS after inducing somatic pain with formalin. Male S.D. rats were injected subcutaneously with 0.1 ml of 5% formalin in the plantar surface of right hindpaw. For experimental group, aspirin was administered orally before injection of formalin. Asprin -untreated group was utilized as the control group. Rats were sacrificed at 0.5, 1, 2, 6 and 24 hours after formalin injection. Rat brains were removed and sliced in rat brain matrix. Brain slices were coronally sectioned at interaural 5.70 ~6.70 mm. Serial sections were immunohisto-chemically reacted with polyclonal c -Fos antibody. The numbers of c -Fos protein immunoreactive neurons in the cingulate cortex, primary somatosensory area, and hippocampus were counted and analyzed statistically with Mann - Whitney U test. Results were as follows: 1. Higher numbers of c -Fos immunoreactive neurons were found in the cingulate cortex, primary somatosensory area and hippocampus. 2. Both aspirin -treated and -untreated groups, numbers of c -Fos immunoreactive neurons were significantly higher all time points than formalin -untreated group, which peacked at 2 hours. 3. The numbers of c -Fos immunoreactive neuron of the aspirin -treated group were less compared to the aspirin - untreated group at each time point. In conclusion, these results provide some basic knowledge in understanding the mechanism and control of formalin - induced somatic pain.
