Exploration of Combinational Quality Control Method of Carbonized Ginger Based on HPLC Fingerprint and QAMS
10.13422/j.cnki.syfjx.20192249
- VernacularTitle:HPLC指纹图谱与一测多评法相结合的姜炭质量控制方法探索
- Author:
Hai-pei SHI
1
;
Hui YAN
1
;
Mei-yu SHEN
1
;
Jia-li WANG
1
;
Sheng-liang HUANG
2
;
Wei DONG
2
;
Guo-qiang WANG
2
;
Bei-hua BAO
1
;
Li ZHANG
1
Author Information
1. Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China
2. Jiangsu Rongyu Pharmaceutical Co. Ltd., Huaian 223001, China
- Publication Type:Research Article
- Keywords:
carbonized ginger;
decoction pieces;
high performance liquid chromatography;
fingerprint;
quantitative analysis of multi-components by single marker;
relative correction factor;
quality control
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(5):95-100
- CountryChina
- Language:Chinese
-
Abstract:
Objective::To establish the HPLC fingerprint of carbonized ginger and to determine the contents of zingerone, 6-gingerol, 6-shogaol, 10-gingerol, 8-shogaol and 10-shogaol with quantitative analysis of multi-components by single marker (QAMS). Method::The fingerprint of carbonized ginger was established by HPLC. All samples were analyzed by Waters SymmetryShield™ RP18 column (4.6 mm×250 mm, 5 μm) with gradient elution by acetonitrile(A)-water(B) (0-30 min, 25%-70%A; 30-50 min, 70%-90%A; 50-60 min, 90%A), the flow rate was 1.0 mL·min-1, the detection wavelength was set at 240 nm and the column temperature was 30 ℃. Zingerone, 6-gingerol, 8-gingerol, 6-shogaol, 10-gingerol, 8-shogaol and 10-shogaol was chosen as marker ingredients to establish HPLC fingerprint of carbonized ginger decoction pieces. Taking 6-gingerol as internal reference standard, the contents of zingerone, 6-shogaol, 10-gingerol, 8-shogaol and 10-shogaol were determined at the detection wavelength of 220 nm and 280 nm according to the relative correction factor. Result::The HPLC fingerprint of carbonized ginger was obtained and 10 common peaks were designated, and 7 of them were identified as zingerone, 6-gingerol, 8-gingerol, 6-shogaol, 10-gingerol, 8-shogaol and 10-shogaol, respectively. And there were no significant differences between the quantitative results of external standard method and QAMS. It is suggested that the content limits of carbonized ginger should be not less than 0.020%of zingerone (C11H14O3), 0.050%of 6-gingerol (C17H26O4), 0.120%of 6-shogaol (C17H24O3), 0.080%of 10-gingerol (C21H34O4), 0.030%of 8-shogaol (C19H28O3) and 0.050%of 10-shogaol (C21H32O3) calculated with reference to the dried products, respectively. Conclusion::The developed method is accurate and feasible, which can provide a simple and effective method for the quality control of carbonized ginger.
