Effect of Serum Containing Yanghetang on Apoptosis of MCF-7 Cells in Breast Cancer Through p38/STAT3 Signaling Pathway
10.13422/j.cnki.syfjx.20192222
- VernacularTitle:阳和汤含药血清通过p38/STAT3信号通路对乳腺癌MCF-7细胞凋亡的影响
- Author:
Shuo YANG
1
;
Kang-le LI
2
;
Zhong-bo ZHU
1
;
Qian HUANG
3
;
Jia YAO
3
;
Yuan ZHI
3
;
Jian-wei DOU
2
Author Information
1. School of Basic Medicine, Gansu University of Chinese Medicine, Lanzhou 730000, China
2. School of Pharmacy, Xi' an Jiaotong University, Xi'an 710061, China
3. Xi' an Hospital of Traditional Chinese Medicine, Xi'an 710021, China
- Publication Type:Research Article
- Keywords:
Yanghetang;
drug containing serum;
MCF-7 cells in breast cancer;
mitogen-activated protein kinase (p38)/signal transduction and transcriptional activator 3 (STAT3)
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(5):6-10
- CountryChina
- Language:Chinese
-
Abstract:
Objective::To investigate the effect of serum containing Yanghetang (YHT) on the apoptosis of MCF-7 cells in breast cancer based on the mitogen-activated protein kinase (p38)/signal transduction and transcriptional activator 3 (STAT3) signal pathway. Method::YHT liquid with crude drug 1 g·mL-1 was prepared. Female SD rats were randomly divided into control group (distilled water), and high, medium and low-dose YHT groups (24, 12, 6 g·kg-1). YHT-medicated serum was prepared, and 10%medicated serum was used to intervene MCF-7 cells. Cell proliferation and cytotoxicity assay (CCK-8) was used to detect the effect of serum containing YHT on MCF-7 cell proliferation, apoptosis of MCF-7 cells was detected by flow cytometry protein expressions of p38 and STAT3 were detected by Western blot, Quantitative Real-time PCR (Real-time PCR) was used to detect the expressions of B-cell lymphoma/leukemia-xl(Bcl-xl) and Survivin mRNA. Result::CCK-8 assay showed that YHT serum inhibited the proliferation of MCF-7 cells in a time and dose-dependent manner compared with the blank group. The inhibitory effect was most obvious in the high-dose group, with the inhibition rates of 38%, 45%and 54%at different time points (P<0.01). Flow cytometry showed that, compared with the blank group, the apoptosis rate in the medium and high-dose groups increased significantly in a dose-dependent manner, with the apoptosis rates at 11.6%and 16.5%respectively (P<0.05, P<0.01). Western blot analysis showed that, compared with the blank group, the expressions of p38 and STAT3 protein was decreased in high, medium-dose YHT groups (P<0.01) in a dose-dependent manner. Compared with the blank group, the expressions of Bcl-xl and Survivin mRNA were decreased in high, medium-dose YHT groups (P<0.05, P<0.01) in a dose-dependent manner. Conclusion::YHT serum can promote the apoptosis of MCF-7 cells in breast cancer, which may be related to the p38/ STAT3 signaling pathway.
