Analysis on Quality Evaluation Methods of Asparagi Radix Decoction Pieces and Its Standard Decoction
10.13422/j.cnki.syfjx.20201064
- VernacularTitle:天冬饮片与标准汤剂的质量评价方法探索
- Author:
Ru-na JIN
1
;
Li-xia HAO
2
;
Tao WANG
1
;
Hong-hua WU
1
;
Yun-tao DAI
2
;
Shou-gang SHI
3
;
Zheng-jun HUANG
3
Author Information
1. Institute of Traditional Chinese Medicine (TCM),Tianjin University of TCM,Tianjin 300193,China
2. Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences, Beijng 100700,China
3. Sunflower Pharmaceutical Group (Xiangyang) Longzhong Co. Ltd.,Xiangyang 441003,China
- Publication Type:Research Article
- Keywords:
Asparagi Radix;
decoction pieces;
standard decoction;
protodioscin;
protoneodioscin;
fingerprint;
high performance liquid chromatography-evaporative light scattering detection method (HPLC-ELSD)
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(17):111-118
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish the quality evaluation methods of Asparagi Radix decoction pieces and its standard decoction. Method:Ten batches of Asparagi Radix standard decoction were prepared. High performance liquid chromatography-evaporative light scattering detection method (HPLC-ELSD) was established for the determination of protodioscin and protoneodioscin in Asparagi Radix decoction pieces and its standard decoction, and the fingerprint detection of Asparagi Radix decoction pieces with acetonitrile-water as mobile phase for gradient elution. UHPLC-LTQ-Orbitrap-MS/MS was used to identify ten main common peaks in the fingerprint with acetonitrile-0.1% formic acid solution as mobile phase for gradient elution, electrospray ionization (ESI) and positive and negative ion mode scanning were employed, the detection range was m/z 100-1 400. Result:The total content of protodioscin and protoneodioscin in Asparagi Radix decoction pieces was 0.41%-0.72%, and their total content in Asparagi Radix standard decoction was 0.33%-0.59%, the transfer rate of these two components was 73.6%-98.3%. The dry extract yield of the standard decoction was 59.0%-73.0%, and its pH was 4.9-5.6. There were 10 common peaks in the fingerprint, and all of them were saponins, including protoneodioscin, protodioscin, aspacochioside A and its isomer, methyl protodioscin, asparagoside F, (25R)-26-O-β-D-glucopyranosyl-furostan-5, 20-diene-3β, 26-diol-3-O-[α-L-rhamnopyranosyl (1→2)]-[β-D-glucopyranosyl (1→4)-α-L-rhamnopyranosyl (1→4)]-β-D-glucopyranoside, 26-O-β-D-glucopyranosyl-furostan-20 (22)-ene-3β, 26-diol-3-O-[α-L-rhamnopyranosyl (1→2)]-[α-L-rhamnopyranosyl (1→4)]-β-D-glucopyranoside, pseudodiosgenin, aspacochioside C. Conclusion:In this paper, the quality evaluation methods of Asparagi Radix decoction pieces and its standard decoction are established, and these methods are stable and feasible, which can provide reference for the quality control of pharmaceutical preparations containing Asparagi Radix.
