Effect of Different Concentrations of Astragali Radix Containing Serum on CYP24A1，CYP27B1 mRNA and Protein in Osteogenic Differentiation of Aging Bone Marrow Mesenchymal Stem Cells
10.13422/j.cnki.syfjx.20201738
- VernacularTitle:黄芪含药血清对衰老骨髓间充质干细胞CYP24A1，CYP27B1 mRNA和蛋白的影响
- Author:
Qian LI
1
;
Yong-zhen WU
1
;
Lian-cheng GUAN
2
;
Jie GAO
1
;
Wen LI
1
;
Zhong QIN
1
;
Yun-zhi CHEN
1
;
Yi-Hui CHAI
1
Author Information
1. Guizhou University of Traditional Chinese Medicine（TCM），Guiyang 550025，China
2. The Second Affiliated Hospital of Guizhou University of TCM，Guiyang 550002，China
- Publication Type:Research Article
- Keywords:
bone marrow mesenchymal stem cells（BMSCs）;
Astragali Radix containing serum;
osteoporosis;
24-hydroxylase（CYP24A1）;
1α-OHase（CYP27B1）
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(17):49-55
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of different concentrations of Astragali Radix containing serum on the expression of 24-hydroxylase（CYP24A1），1α-OHase（CYP27B1） mRNA and protein in rat bone marrow mesenchymal stem cells （BMSCs）， and to explore the mechanism of primary osteoporosis （OP）. Method:The experiment was divided into 6 groups，like normal group， model group， low ，middle and high dose group of Astragali Radix containing serum（20%，40%，60%），Vitamin D group. Cell proliferation toxicity assay（CCK-8） was used to detect the effect of different concentrations of Astragali Radix containing serum on survival rate of aging BMSCs.Real-time quantitative PCR（Real-time PCR） and Western blot was used to detect the expression of CYP24A1 CYP27B1 mRNA and protein in senile BMSCs osteogenic differentiation cells by different concentrations of Astragali Radix containing serum. Result:Compared with normal group， the proliferation and survival rate of BMSCs osteoblasts induced by D-galactose in model group was significantly lower than that in normal group （P<0.01）. Compared with model group， medium and high dose groups and Vitamin D group could improve the proliferation and differentiation of aging BMSCs into osteoblasts in different degrees（P<0.01）. The relative expression of CYP27B1 mRNA and protein in model group was significantly lower than that in normal group， while the relative expression of CYP24A1 mRNA and protein in model group was significantly higher than that in normal group. Compared with model group， high dose Astragali Radix containing serum group could increase the relative expression of CYP27B1 mRNA and protein， and decrease the relative expression of CYP24A1 mRNA and protein in a dose-dependent manner（P<0.01）. Conclusion:The mechanism of different concentrations of Astragali Radix containing serum in the treatment of osteoporosis may be related to the regulation of CYP24A1， CYP27B1 mRNA and protein in the osteogenic differentiation of aging BMSCs.
