Effect of Xihuangwan on NLRP3 Inflammatory Bodies and Their Products and Tumor Proliferation of Lung Cancer A549 Bearing Nude Mice in Inflammatory Microenvironment
10.13422/j.cnki.syfjx.20201722
- VernacularTitle:西黄丸对炎症微环境下肺癌A549荷瘤小鼠NLRP3炎症小体及其产物和肿瘤增殖的影响
- Author:
Rui-yuan JIANG
1
;
Chun-mei MO
2
;
Ting-ting MAN
1
;
Tong-biao WANG
2
;
Xiao-hua HONG
1
;
Yan-chun QIN
1
;
Zhen RONG
2
Author Information
1. Graduate School，Guangxi University of Chinese Medical，Nanning 530200，China
2. The First Affiliated Hospital of Guangxi University of Chinese Medical，Nanning 530200，China
- Publication Type:Research Article
- Keywords:
Xihuangwan;
A549 lung cancer bearing nude mice;
nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3);
inflammatory microenvironment;
proliferation inhibition
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(17):20-28
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the antitumor effect of Xihuangwan on A549 lung cancer nude mice in inflammatory microenvironment, and explore the effect of Xihuangwan on the expressions of nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3) inflammatory bodies and their products in serum and tumor tissue of A549 lung cancer nude mice. Method:The lung cancer A549 cell model was established in nude mice with lung cancer, and the lung cancer A549 cell model was established in inflammatory microenvironment by adding lipopolysaccharide (LPS) + adenosine triphosphate (ATP) to the culture medium. After modeling, the rats were randomly divided into blank group (equal volume of normal saline), positive drug control group (MCC950 solution, 0.79 g·kg-1), and low, medium, high-dose Xihuangwan groups (0.39, 0.78, 1.95 g·kg-1). The rats were administered orally once a day for 21 days, and then sacrificed. The tumor tissues were stripped to measure the tumor body. The expressions of NLRP3, malondialdehyde（MDA）, interleukin (IL)-1β, IL-18 and NLRP3 were detected by Western blot, and the levels of IL-1β, IL-18 and MDA were detected by enzyme linked immunosorbent assay（ELISA）. Result:Compared with the blank group, the tumor inhibition rates in the positive drug control group and the low, medium and high dose Xihuangwan group were 39.21%, 31.72%, 42.24% and 55.68%. ELISA showed that the high-dose Xihuangwan group could significantly reduce the expressions of MDA, IL-1β and IL-18 in the serum of nude mice (P<0.05). Western blot showed that the high-dose Xihuangwan group could effectively reduce the protein expressions of MDA, IL-1β, IL-18 and NLRP3 in tumor of nude mice (P<0.05), the results of immunohistochemistry showed that the expression rate of NLRP3 in the tumor tissues of nude mice was reduced in the positive drug group and each dose of Xihuangwan group (P<0.05). Conclusion:Xihuangwan can inhibit the growth of tumor tissue of A549 cells bearing lung cancer in nude mice. The mechanism may be that it can inhibit the growth of tumor cells by inhibiting the expression of NLRP3 inflammatory bodies, IL-1β, IL-18, MDA tables, and then inhibiting the inflammatory microenvironment of tumor cells.
