Identification of Chemical Constituents in Caulis of Erycibe schmidtii by UPLC-Q-Exactive Focus-MS/MS
10.13422/j.cnki.syfjx.20201162
- VernacularTitle:光叶丁公藤中化学成分的UPLC-Q-Exactive Focus-MS/MS鉴定
- Author:
Jing HU
1
;
Yuan-yuan YANG
2
;
Hui REN
1
;
Xiao-min CUI
1
;
Xiao-mei LIU
1
;
Jia-yi LUO
3
;
Zhi-yong CHEN
1
Author Information
1. Shaanxi Academy of Traditional Chinese Medicine,Xi'an 710061,China
2. Xi'an Institute for Food and Drug Control,Xi'an 710054,China
3. Medical School, Xi'an Peihua University,Xi'an 710125,China
- Publication Type:Research Article
- Keywords:
Erycibe schmidtii caulis;
ultra-high performance liquid chromatography coupled with Q-Exactive Focus mass spectrometry (UPLC-Q-Exactive Focus-MS/MS);
chlorogenic acids;
coumarins;
tropane alkaloids;
amides;
esterified glycosides
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(18):124-132
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To rapidly recognize and identify the chemical constituents in caulis of Erycibe schmidtii by ultra-high performance liquid chromatography coupled with Q-Exactive Focus mass spectrometry (UPLC-Q-Exactive Focus-MS/MS). Method:Taking 80% methanol extract of E. schmidtii caulis as the test solution, the chemical constituents in caulis of E. schmidtii were analyzed and identified. Thermo Accucure aQ C18 column (2.1 mm×150 mm, 2.6 μm) was used for chromatographic separation with the mobile phase of methanol (A)-0.1% formic acid solution (B) for gradient elution (0-12 min, 5%-25%A; 12-20 min, 25%-30%A; 20-28 min, 30%-38%A; 28-40 min, 38%-42%A). Positive and negative ion monitoring modes and heated electrospray ion source (HESI) were used for mass spectrographic analysis. The scanning range was m/z 80-1 200. Result:A total of 42 chemical constituents from caulis of E. schmidtii were identified, including 12 coumarins, 14 chlorogenic acids, 1 tropane alkaloid, 1 amide and 14 esterified glycosides. Conclusion:Chemical constituents in caulis of E. schmidtii can be quickly and fully identified by UPLC-Q-Exactive Focus-MS/MS. Among them, 11 compounds are unambiguously identified by comparing with reference standards, 31 compounds are reported for the first time in this herb, 2 compounds are reported for the first time in Erycibe plants. This paper can provide the important basis for study on pharmacodynamic material base and substitute development of E. schmidtii caulis.
