Huidouba Ameliorates Kidney Oxidative Stress Injury by Down-regulating Nox4 Expression in Rats with Diabetic Nephropathy
10.13422/j.cnki.syfjx.20201839
- VernacularTitle:藏族药灰兜巴下调Nox4表达减轻糖尿病肾病大鼠肾脏氧化应激损伤
- Author:
Kun-bao YANG
1
;
Zong-ran PANG
1
;
Ying-hui BAI
1
;
Bi-nan LU
1
;
Ning YU
2
;
Gui-yan HAN
2
;
Chang-jiang YIN
2
Author Information
1. College of Pharmacy, Minzu University of China,Beijing 100081,China
2. Chengde Medical University,Chengde 067000,China
- Publication Type:Research Article
- Keywords:
Huidouba;
nicotinamide adenine dinucleotide phosphate （NADPH） oxidase 4（Nox4）;
proteinuria;
diabetic nephropathy;
oxidative stress
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(18):84-90
- CountryChina
- Language:Chinese
-
Abstract:
Objective:From a new perspective，to explore therapeutic effect of Huidouba (HDB) on alleviating kidney oxidative damage in rats with diabetic nephropathy (DN) and provide a scientific basis for developing HDB as a potential Tibetan medicine for treatment of DN. Method:Rats were fed with high-fat diet (HFD) and injected with streptozocin (STZ, 65 mg·kg-1) intraperitoneally to induce DN model, while rats in Blank group were injected with an equal volume of vehicle and fed with normal chow. The successfully modeling DN rats were randomly divided into three groups, 8 rats per group, DN model group (10 mL·kg-1·d-1), Metformin group (0.045 g·kg-1·d-1) and HDB group (0.18 g·kg-1·d-1). Monitor body weight (BW) and fasting blood glucose (FBG) weekly, and collect 24 hours urine before and after medication to examine microalbuminuria (mAlb). Calculate kidney index (KI) after sacrificing, analyze mAlb, serum creatinine (SCr) and blood urea nitrogen (BUN) with a fully automatic biochemical analyzer. Histopathology of kidney was observed by Masson staining. Lipid peroxidation malondialdehyde (MDA) assay kit was used to examine MDA content in kidney tissue. Nox4, as a subtype of triphosphopyridine nucleotide (NADPH) oxidase family was determined by Western blot and immunofluorescence assay of kidney tissue. Result:Compared with blank group, levels of FBG, 24 h mAlb, SCr, BUN and MDA in DN model group were increased (P<0.01), tissue damage was obvious and Nox4 expression in glumeruli was increased significantly (P<0.01). Compared with DN model group, levels of FBG, 24 h mAlb, SCr, BUN and MDA in drug administration groups were decreased (P<0.01), kidney injury was alleviated and Nox4 expression was down-regulated（P<0.01）. Conclusion:HDB as a Yiqiyangyin Tibetan medicine, could ease oxidative stress injury of kidney and reduce proteinuria in DN rats, thus prevent the development of DN. Its mechanism is closely related to down-regulating Nox4 expression of kidney tissue in DN rats.
