Isolation and Identification of Mycena of Pleurotus in Wumeng Mountain Area and Optimization of Its Production Conditions
10.13422/j.cnki.syfjx.20202068
- VernacularTitle:乌蒙山区小菇属萌发菌的分离鉴定及其菌种生产优化
- Author:
Hui WANG
1
;
Qing-song YUAN
1
;
Tao ZHOU
1
;
Yuan YUAN
2
;
Jiao XU
1
;
Wei-ke JIANG
1
;
Jin-qiang ZHANG
1
;
Xiao WANG
3
;
Da-hui LIU
4
;
Guang-wen ZHANG
5
;
Cheng PAN
5
Author Information
1. Guizhou University of Traditional Chinese Medicine（TCM），Guiyang 550025，China
2. National Resource Center for Chinese Meteria Medica，Chinese Academy of Chinese Medical Sciences，Beijing 100700，China
3. Shandong Analysis and Test Center，Ji'nan 250014，China
4. Hubei University of Chinese Medicine，Wuhan 430065，China
5. Guizhou Wumengteng Fungus Industry Limited Company，Dafang 551600，China
- Publication Type:Research Article
- Keywords:
Gastrodia elata;
germination;
Mycena;
separation;
identification
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(19):43-52
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Isolate and identify Mycena, expand the resources of geminating fungus of Gastrodia elata and optimize the culture conditions of Mycena，in order to provide information and guidance for the production of geminating fungus of G. elata. Method:Juvenile tuber tissue mass transfer separation and purification technology was used for the separation and purification of strains，traditional morphology microscopy was used to isolate the colony mycelia spores morphological characteristics, such as identification，polymerase chain reaction（PCR） amplification rDNA （Ribosomal DNA） internal transcribed spacer（ITS） was used for sequencing analysis and further homology with NCBI database retrieval，MEGA6 software was used to establish Phylogenetic tree by the Maximum likelihood method （MaximumLikelihood，M-L）, so as to classify and identify isolated strains. At the same time，orthogonal test was used to optimize the optimal growth conditions of Mycena. Result:A total of 86 strains were isolated, which belong to 21 species in 12 genera. WMMFJ，SHXG，WMM-21 and MFJ8103 were identified as M. purpureofusca, and ZT01-6 and ZT01-8 were identified as M. cf. purpureofusca. The growth rate of Mycena in wheat bran medium was significantly higher than in PDA medium. The optimal medium composition for the growth of germinating bacteria was 100 g potato，150 g wheat bran and 20 g corn flour，100 g glucose. And 1，3，5-Trihydroxybenzene significantly promoted the growth of WMMFJ，and played a role in promoting the growth of WMM-21 and ZT01-6，and 2-Methoxyphenol promoted the growth of WMMFJ. Conclusion:Six strains of Mycena were isolated and identified，four of them are M. purpureofusca，and two of them are M. cf. Purpureofusca. The separation method improved the separation effect of germinating bacteria.
