Effects of High Temperature Stress on Growth Characteristics of Armillaria
10.13422/j.cnki.syfjx.20202017
- VernacularTitle:高温胁迫对蜜环菌生长特性的影响
- Author:
Xuan HUANG
1
;
Jiao XU
1
;
Tao ZHOU
1
;
Xiao-hong OU
1
;
Qing-song YUAN
1
;
Jin-qiang ZHANG
1
;
Wei-ke JIANG
1
;
Yuan YUAN
2
;
Xiao WANG
3
;
Da-hui LIU
4
;
Guang-wen ZHANG
5
Author Information
1. Guizhou University of Traditional Chinese Medicine，Guiyang 550025，China
2. National Resource Center for Chinese Meteria Medica，Chinese Academy of Chinese Medical Sciences，Beijing 100700，China
3. Shandong Analysis and Test Center，Ji'nan 250014，China
4. Hubei University of Chinese Medicine，Wuhan 430065，China
5. Guizhou Wumengteng Fungus Industry Limited Company，Dafang 551600，China
- Publication Type:Research Article
- Keywords:
Armillaria;
Gastrodia elata;
high temperature stress;
growth characteristics
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(19):17-22
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effects of high temperature stress on the growth characteristics of different Armillaria strains，and to provide guidance for screening excellent Armillaria strains with high-temperature resistance. Method:14 strains of Armillaria from different G. elata producing areas were used as experimental materials to observe the growth characteristics and conduct phenotypic classification for the strains. rDNA-IGS sequence analysis was used for molecular identification to further determine the genetic relationship of the tested strains.The strain growth rate, biomass，mycelial length and other indicators under the condition of 23 ℃ （CK） and 30 ℃ high temperature stress were recorded. Result:All the 14 strains of Armillaria had the highest similarity and the closest relationship with Armillaria gallica，but there were significant differences in growth characteristics among different G. elata producing areas. The 14 strains of Armillaria were classified into Ⅳ groups，and the growth status was groupⅠ>group Ⅱ>group Ⅲ>group Ⅳ. After treatment with high temperature stress，the tolerance of each strain to high temperature also showed obvious differences，as shown in the average growth rate of the mycelial was GZ16>SX1>GZ1. The rank of relative mycelial length was GZ16>SX1>GZ3 and the relative biomass was GZ16>SX4>GZ1>HB1>AH2. Conclusion:Under high temperature stress，GZ16 was best in growth rate，relative length of mycelial，relative biomass and growth state，followed by SX1 and GZ1 strains. The results indicate that strains GZ16，SX1 and GZ1 have the strong resistance to high temperature and excellent growth characteristics at normal temperature，so these three strains are suitable to be produced in main G. elata producing areas in China.
