Effect of Gastrodia elata Planting on Soil Microbial Community Structure
10.13422/j.cnki.syfjx.20202016
- VernacularTitle:天麻种植对土壤微生物群落结构的影响分析
- Author:
Jiao XU
1
;
Xuan HUANG
1
;
Tao ZHOU
1
;
Xiao-hong OU
1
;
Yan-hong WANG
1
;
Wei-ke JIANG
1
;
Yuan YUAN
2
;
Da-hui LIU
3
;
Xiao WANG
4
;
Guang-wen ZHANG
5
Author Information
1. Guizhou University of Traditional Chinese Medicine，Guiyang 550025，China
2. National Resource Center for Chinese Meteria Medica，Chinese Academy of Chinese Medical Sciences，Beijing 100700，China
3. Hubei University of Chinese Medicine，Wuhan 430065，China
4. Shandong Analysis and Test Center，Ji'nan 250014，China
5. Guizhou Wumengteng Fungus Industry Limited Company，Dafang 551600，China
- Publication Type:Research Article
- Keywords:
Gastrodia elata;
Armillaria;
soil microbial;
diversity;
abundance
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(19):10-16
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the changes of soil microbial community structure before and after planting Gastrodia elata in different producing areas，and to investigate the response of soil microorganisms to the planting of G. elata. Method:ITS and 16S rDNA high-throughput sequencing technologies were used to detect fungal and bacterial community compositions in the soil，including the soil without planting G. elata（CK1，CK2），the soil around G. elata（GE1，GE2）before harvesting, and the soil around the rhizomorph of Armillaria（AGE1，AGE2） in Dafang, Guizhou and Jinzhai, Anhui respectively. Result:Principal component analysis (PCA) showed that the soil microorganisms changed significantly after G. elata planting as compared with the control soil. The sequencing results showed that the planting of G. elata increased the OTUs number of fungi and bacteria. As compared with the control soil，the diversity and abundance of fungal and bacterial communities showed an increase trend after the cultivation of G. elata in soil of Dafang, Guizhou， such changes of fungal communities were not significant, but the abundance of soil bacteria communities increased in Jinzhai, Anhui as compared with the control soil. The abundance of genera Ilyonectria and Nitrospira increased，while genera Russula decreased significantly both in the soil of Guizhou and Anhui. Furthermore，the abundance of Fusarium and Mortierella increased significantly in the soil of Dafang, Guizhou. Conclusion:The soil microorganisms were out of balance after planting of G. elata, and the abundance of pathogenic microorganisms such as Ilyonectria and Fusarium increased，which may be related to the plant diseases and insect pests of G. elata.
