Systematic Identification and Quality Evaluation of Anemonis Flaccidae Rhizoma from 16 Different Places of Origin
10.13422/j.cnki.syfjx.20201947
- VernacularTitle:16个产地地乌药材的系统鉴定及质量评价
- Author:
Xin HU
1
;
Xu WANG
1
;
Man LIU
1
;
Hong PEI
2
;
Zhi-guo ZHU
3
;
Bi-sheng HUANG
1
;
Yi-fei LIU
1
;
Cheng-wu SONG
1
;
Zhi-gang HU
1
Author Information
1. Pharmacy Faculty,Hubei University of Chinese Medicine,Wuhan 430065,China
2. GKH Pharmaceutical Co. Ltd.,Guangzhou 511440,China
3. Hubei Jingui Chinese Medicine Pieces Co. Ltd.,Wuhan 430051,China
- Publication Type:Research Article
- Keywords:
Anemonis Flaccidae Rhizoma;
traditional authentication;
DNA barcoding;
ribosomal DNA internal transcribed spacer 2 (ITS2);
high performance liquid chromatography (HPLC);
quality evaluation;
origin selection
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(20):132-139
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To construct a systematic identification system of Anemonis Flaccidae Rhizoma, and to evaluate the comprehensive quality of Anemonis Flaccidae Rhizoma from 16 regions in China, so as to lay a foundation for its origin selection and clinical medication safety. Method:The authenticity of Anemonis Flaccidae Rhizoma was quickly identified by traditional identification method and DNA barcode molecular identification technology, and HPLC-UV was used to determine the contents of 5 active ingredients in Anemonis Flaccidae Rhizoma. All high pressure chromatographic separations were performed with a Welch Ultimate XB-C18 column (4.6 mm×250 mm, 5 μm), the mobile phase consisted of acetonitrile-0.01% trifluoroacetic acid aqueous solution (30∶70) at a flow rate of 1.0 mL·min-1. The detection wavelength was set at 210 nm and the column temperature was maintained at 30 ℃. Result:The authenticity of Anemonis Flaccidae Rhizoma could be precisely and rapidly identified by ribosomal DNA internal transcribed spacer 2 (ITS2) sequence and traditional identification methods. BLAST comparative analysis found that medicinal materials from 16 areas were all Anemone flaccida. Based on the contents of multi-index components, it was shown that the total content of 5 triterpenoid saponins in Anemonis Flaccidae Rhizoma from Banqiao, Enshi, Hubei was the highest (10.59%), followed by Hezhang, Bijie, Guizhou (6.28%) and Duzhenwan, Changyang, Hubei (5.64%). Conclusion:DNA barcoding can be used as an effective supplement to the traditional identification technology, it can ensure the authenticity of Anemonis Flaccidae Rhizoma and the safety of clinical use. The comprehensive evaluation of multi-index components of HPLC and cluster analysis show that the quality of medicinal materials in Enshi, Changyang, Wufeng of Hubei, Bijie of Guizhou and Jinfoshan of Chongqing is superior, which can be considered as important origin of Anemonis Flaccidae Rhizoma.
