Effect of Plantaginis Semen on Inflammatory Cytokines and mRNA and Protein Expressions of AQP4 in Colon Tissue of Diarrhea Rats
10.13422/j.cnki.syfjx.20201902
- VernacularTitle:车前子对腹泻大鼠炎性因子和结肠组织AQP4 mRNA和蛋白表达的影响
- Author:
Yue WANG
1
;
Ya-ning BIAO
1
;
Xin-qiao CHU
1
;
Na-bo ZHANG
1
;
Mu-qing ZHANG
2
;
Xi WANG
1
;
Xue HAN
1
;
Yi-xin ZHANG
1
Author Information
1. School of Pharmacy, Hebei University of Chinese Medicine, Application Technology Research Center of Traditional Chinese Medicine(TCM) Prescription in University of Hebei Province, Shijiazhuang 050200, China
2. Hebei Hospital of TCM, Shijiazhuang 050011, China
- Publication Type:Research Article
- Keywords:
diarrhea;
Plantaginis Semen;
tumor necrosis factor-α (TNF-α);
interleukin-6 (IL-6);
C-reactive protein (CRP);
aquaporin4 (AQP4)
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(20):52-58
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe Plantaginis Semen's mechanism in treating diarrhea by observing the effect on inflammatory factors in serum and mRNA and protein expressions of aquaporin4 (AQP4) in colon tissue of diarrhea rats. Method:Senne Folium was orally administered to duplicate diarrhea rats. Sixty male SD rats were randomly divided into normal group, model group, hydrochlorothiazide group (9 mg·kg-1), and low, middle, and high-dose Plantaginis Semen groups (0.95, 1.9, 3.8 g·kg-1). Senne Folium (20 mL·kg-1) was intragastrically administered in 5 groups in the morning, except for normal group that was orally given the same dose of distilled water. In the afternoon, each treatment group was orally given the corresponding drugs, while normal group and model group were orally given the same dose of distilled water. The loose stool rate, average degree of loose stool, and diarrhea index were compared according to fecal traits and stool times after 14 days of treatment. The serum and colon tissue were collected to detect the contents of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and C-reactive protein (CRP) in serum. Hematoxylin-eosin (HE) staining was used to observe the pathological morphological changes of colon tissue, and quantiative Real-time fluorescent quantitative polymerase chain reaction (Real-time PCR) and Western blot were used to detect the mRNA and protein expressions of AQP4 in colon tissue. Result:In the model group, the loose stool rate, average degree of loose stool, and diarrhea index were significantly increased (P<0.01), apoptosis and necrosis were observed in the epidermal cells of colonic mucosa, telangiectasia and congestion in lamina propria were obvious, and a few neutrophils were infiltrated, and the contents of TNF-α, IL-6 and CRP in serum increased (P<0.05, P<0.01), the mRNA and protein expressions of AQP4 significantly decreased (P<0.01). Compared with the model group, the loose stool rate, average degree of loose stool, and diarrhea index were significantly decreased in low, middle, and high-dose Plantaginis Semen groups (P<0.01), the apoptosis and necrosis of epidermal cells, telangiectasia and hyperemia and neutrophil infiltration in colonic mucosa were obviously improved, and the contents of TNF-α and CRP in serum significantly decreased (P<0.05, P<0.01), the mRNA and protein expressions of AQP4 increased （P<0.05, P<0.01）. Conclusion:Plantaginis Semen has a better antidiarrheal effect, and its mechanism may be related to inhibition of inflammatory reaction, repair of pathological damage of colonic mucosa, up-regulation of AQP4 expression and promotion of water and fluid metabolism.
