Effect of Euphorbia helioscopia Alcohol Extract on MAPK/NF-κB Inflammation Pathway on Mice with Acute Lung Injury Induced by LPS
10.13422/j.cnki.syfjx.20202039
- VernacularTitle:泽漆醇提物对LPS诱导小鼠急性肺损伤及其炎症信号通路MAPK/NF-κB的影响
- Author:
Lan-ying CHEN
1
;
Li YIN
1
;
Meng-jing ZHOU
1
;
Feng SHAO
2
;
Qiang SHI
3
;
Rong-hua LIU
2
Author Information
1. National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine， Jiangxi University of Traditional Chinese Medicine（TCM）,Nanchang 330006,China
2. School of Pharmacy, Jiangxi University of TCM,Nanchang 330004,China
3. Qihuang Academy of TCM, Jiangxi University of TCM,Nanchang 330025,China
- Publication Type:Research Article
- Keywords:
Euphorbia helioscopia alcohol extracts;
lipopolysaccharide;
acute lung injury;
inflammation;
mitogen-activated protein kinase /nuclear factor kappa-B (MAPK/NF-κB) pathway
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(20):46-51
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the protective effect of Euphorbia helioscopia alcohol extract on lipopolysaccharide (LPS) -induced acute lung injury in mice and explore its possible mechanism. Method:The 50 Balb/c male mice were randomly divided into 5 groups, including normal group, model group, dexamethasone group (1.5 mg·kg-1), E. helioscopia alcohol extracts group (7.5，3.75 g·kg-1). Except for the normal group, the other groups used intranasal instillation of LPS to establish a model of acute lung injury in mice. The type and number of inflammatory cells in bronchoalveolar lavage fluid (BALF) were detected by automatic blood analyzer and Wright-Giemsa composite staining. The lung tissue damage was observed by hematoxylin-eosin (HE) staining. The contents of the inflammatory factors tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in BALF were detected by flow cytometry. The protein expressions of nuclear factor kappa-B p65(NF-κB p65), phospho-NF-κB p65 (p-NF-κB p65), inhibitor of NF-κBα (IκBα), phospho-IκBα (p-IκBα) in NF-κB pathway and c-Jun N-terminal kinase (JNK), phospho-JNK (p-JNK), p38 protein (p38), phospho-p38 (p-p38), extracellular regulated protein kinases (ERK1/2), phospho-ERK1/2 (p-ERK1/2) in mitogen-activated protein kinase (MAPK) pathway were determined by Western blot. Result:Compared with normal control group, the lung tissue of the model group showed obvious damage, in which a large number of inflammatory cells infiltrated, and the integrity of the alveoli was destroyed. Inflammatory factors TNF-α, IL-6 in BALF and p-NF-κB p65, p-JNK, p-p38, p-ERK protein expression levels in lung tissue were significantly increased (P<0.01). Compared with model group, the pathological damage of lung tissue in mice with high dose of E. helioscopia alcohol extract and dexamethasone positive group was significantly alleviated. The levels of TNF-α and IL-6 in BALF and the expression levels of p-NF-κB p65, p-JNK, p-p38 and p-ERK1/2 protein in lung tissue were significantly down-regulated (P<0.01). Conclusion:The E. helioscopia alcohol extract has a protective effect on LPS-induced acute lung injury in mice, its mechanism may be related to the regulation of the NF-κB/MAPK signaling pathway.
