Identification of Prototype Compounds and Their Metabolites in Rat Plasma After Oral Administration of Aurantii Fructus Extract by UPLC-Q-TOF/MS
10.13422/j.cnki.syfjx.20201555
- VernacularTitle:UPLC-Q-TOF/MS鉴定大鼠灌服枳壳提取物后的入血成分及其代谢产物
- Author:
Fei-ting WEI
1
;
Hao CHENG
1
;
Ri-fa QIAO
1
;
Min ZHANG
1
;
Min-yong ZHONG
1
;
Wu-liang YANG
1
;
Jin-bin YUAN
1
Author Information
1. （Key Laboratory of Modern Preparation of Traditional Chinese Medicine （TCM），Ministry of Education，School of Basic Medical Sciences，Jiangxi University of TCM，Nanchang 330004，China）
- Publication Type:Research Article
- Keywords:
Aurantii Fructus;
serum pharmacochemistry;
ultrahigh performance liquid chromatography-quadrupole-time-of-flight tandem mass spectrometry （UPLC-Q-TOF/MS）;
prototype components;
metabolites;
dihydroflavones;
polymethoxyflavonoids
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(21):161-172
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the serum pharmacochemistry of Aurantii Fructus （AF）， and to investigate the pharmacological material basis of AF extract in rats. Method:Rapid identification and speculation of the prototype constituents and their metabolites in vivo were carried out according to the relative retention time， accurate relative molecular mass， cleavage fragments of MS/MS and neutral loss of metabolites with ultrahigh performance liquid chromatography-quadrupole-time-of-flight tandem mass spectrometry （UPLC-Q-TOF/MS） technique by comparing the differences between different samples such as AF extracts， blank plasma， and administered plasma under the same chromatographic and mass spectrometric conditions. Result:After oral administration of the AF extract， 74 transitional constituents absorbed into the blood were detected in serum， in which 49 compounds were prototype constituents and the other 25 were metabolites. The prototype constituents could be divided into dihydroflavones， polymethoxyflavonoids， limonins， coumarins and alkaloids. The identified metabolites included glucuronic acid conjugates， sulfuric acid conjugates， hydroxylated products of flavonoid glycosides and polymethoxyflavonoids， as well as the simultaneous glucuronidation and sulfation products. Conclusion:The constituents absorbed into the blood and their metabolites may be the pharmacodynamic components of AF. Among them， alkaloids， polymethoxyflavonoids and coumarins are mainly introduced into the blood in the prototype form， while naringin and neohesperidin （the index components） exert effect mainly through hydrolysis into aglycones. This work will help to further elucidate the material basis of AF.
