Colorectal Cancer Modeling and Difference Analysis in Cold and Heat Conditions

Hui-xuan LI; Jing-chun Qin; Zhi-hao Lyu; Yu-qi Liang; Bin Wen

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Colorectal Cancer Modeling and Difference Analysis in Cold and Heat Conditions

VernacularTitle:寒热不同宿体大肠癌模型建立及差异分析
Author: Hui-xuan LI ¹ ; Jing-chun QIN ¹ ; Zhi-hao LYU ² ; Yu-qi LIANG ¹ ; Bin WEN ¹
Author Information

1. Science and Technology Innovation Center，Piwei Institute， Guangzhou University of Chinese Medicine， Guangzhou 510405，China
2. The First Clinical Medical College， Guangzhou University of Chinese Medicine， Guangzhou 510000，China
Publication Type:Research Article
Keywords: cold and heat syndrome; colorectal cancer model; adenosine triphosphate （ATPase）; lactate dehydrogenase （LDH）; succinate dehydrogenase （SDH）
From: Chinese Journal of Experimental Traditional Medical Formulae 2020;26(21):109-117
CountryChina
Language:Chinese
Abstract: Objective:To explore the correlation between the occurrence of colorectal cancer and different internal environment （cold and heat）. Method:The 70 Wistar rats （male and female） were randomly divided into blank group （10 cases）， cold model group （30 cases） and heat model group （30 cases）. The cold syndrome model was made by intragastric infusion of cold water （0 ℃） and soaking in cold water （10 ℃）. The heat model was made by ethanol （30%） and capsaicin solution （0.9 g·L^-1）. The blank group was given normal saline by gavage， 10 mL·kg^-1·d^-1， for 5 consecutive weeks. The colorectal cancer model was made by subcutaneous injection of DMH solution in the back of neck in the cold model group and heat model group at the 6^th week， 25 mg·kg^-1，once a week，for 12 consecutive times. During the carcinogenesis， only 30% ethanol solution was given to the heat model group， and the modeling was maintained in cold model group， 10 mL·kg^-1·d^-1， for 38 weeks. The general state of the rats in each group was observed， and the changes of food intake and body weight were measured. At the 27^th， 29^th， 32^th， 35^th and 38^th weeks， samples were collected in batches. Intestinal tissues were subjected to hematoxylin-eosin staining （HE） and detection of sodium， potassium-adenosine triphosphate （Na⁺K⁺-ATP）， calcium， magnesium-adenosine triphosphate （Ca²⁺ Mg²⁺-ATP） activity， lactate dehydrogenase （LDH） activity and succinate dehydrogenase （SDH） activity. Result:The symptoms of hematochezia and ascites in cold model group were earlier than those in heat model group. As compared with the blank group， the food intake and body weight were decreased in cold model group and heat model group. As compared with the blank group， the length of the large intestine was shorter in cold model group at the 32^nd and 35^th week （P<0.05）， the activities of Na⁺ K⁺-ATP， Ca²⁺ Mg²⁺-ATP increased significantly in heat model group at the 27^th， 29^th and 38^th week （P<0.05， P<0.01）， the SDH enzyme activity was decreased in the cold model group at the 29^th and 35^th week （P<0.05， P<0.01）， the SDH enzyme activity was significantly increased in the heat model group at the 38^th week （P<0.01）. At the 27^th week， LDH enzyme activity was significantly reduced in cold model group （P<0.01）. The LDH activity was increased significantly in heat model group at 29^th and 32^nd week （P<0.05， P<0.01）. As compared with the heat model group， the large intestine texture of the cold model rats showed greater brittleness， aggravated fibrosis， more obvious fibroproliferative characteristics， higher tumor incidence， and more serious tumor differentiation. The Na⁺K⁺-ATP， Ca²⁺ Mg²⁺-ATP enzyme activities of the cold model rats were significantly reduced at 27^th， 29^th， and 38^th weeks （P<0.01）， the SDH enzyme activity was significantly reduced in the cold model rats at 29^th and 38^th weeks （P<0.01）， the LDH activity was reduced in the cold model group at 32^nd week （P<0.05）. Conclusion:Cold environment for colorectal cancer promotes tumorigenesis， and the hot environment can also promote tumorigenesis in a later stage.