Quality Control of Dynamic Volatile Components in Lonicerae Japonicae Flos and Lonicerae Flos
10.13422/j.cnki.syfjx.20201047
- VernacularTitle:金银花、山银花动态挥发性成分的质量控制
- Author:
Hai-ying LI
1
;
Qi-meng FAN
1
;
Yu-ting HE
1
;
Peng HE
1
;
Min-cun WANG
1
;
Run-nan LIU
1
;
Zhuo ZHOU
1
;
Xue-bing QIAN
1
;
Xian-ping ZENG
1
;
Mei-feng XIAO
1
;
Fu-yuan HE
1
Author Information
1. College of Pharmacy，Hunan Provincial Key Laboratory of Druggability and Preparation Modification for Chinese Materia Medica，Laboratory of Supramolecular Mechanism and Mathematic-physics Characterization for Traditional Chinese Medicine， Hunan University of Chinese Medicine，Changsha 410208，China
- Publication Type:Research Article
- Keywords:
supramolecular gas evolution "imprinting template" theory;
molecular connectivity index;
total statistical moment;
stability;
Lonicerae Japonicae Flos;
Lonicerae Flos;
volatile oils
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(22):148-155
- CountryChina
- Language:Chinese
-
Abstract:
Objective:The molecular connectivity index method and total statistical moment method were used to control the quality of traditional Chinese medicine （TCM）， and the stability and consistency of volatile components of Lonicerae Japonicae Flos and Lonicerae Flos were clarified. Method:Volatile oils in Lonicerae Japonicae Flos and Lonicerae Flos from different producing areas was extracted for GC-MS determination with electron bombardment ion source， ion source temperature of 230 ℃， and detection range of m/z 35-650. Then National Institute of Standards and Technology （NIST） 05 and ChemicalBook database were used for qualitative analysis of these volatile components， the peak area normalization method was used for quantitative analysis， and the total statistical moment parameters and the zero-order， first-order， second-order， third-order molecular connectivity indexes of the components were calculated. Result:Number of peaks （RSD were 28.5%， 33.4%， respectively）， total zero-order moments （RSD were 55.5%， 128.9%， respectively） and total second-order moments （RSD were 15.3%， 21.5%， respectively） of 10 batches of Lonicerae Japonicae Flos and Lonicerae Flos were unstable， indicating that the types and contents of volatile components fluctuated sharply， but the total first-order moments （RSD were 7.5%， 8.8%， respectively） and the zero-order， first-order， second-order and third-order molecular connectivity indexes （RSD ranged from 8.1% to 10.3% and 4.2% to 5.5%， respectively） were relatively stable， indicating that the overall "imprinting template" of the components was similar. Statistical analysis of each parameter found that there were no significant differences in the number of peaks， total first-order moments and zero-order， first-order， second-order， third-order molecular connectivity indexes between volatile oils from Lonicerae Japonicae Flos and Lonicerae Flos. Conclusion:Under the guidance of supramolecular gas evolution "imprinting template" theory， the molecular connectivity index method and total statistical moment parameters are used to jointly characterize the "imprinting template" of TCM components in vitro， which can control the stability and consistency of TCM quality.
