Phenols and Organic Acids Constituents in Water Decoction of Curcumae Rhizoma-Sparganii Rhizoma Herbal Pair and Single Herb
- VernacularTitle:“莪术-三棱”药对及其单味药水煎液的酚类和有机酸类化学成分分析
- Author:
Xiao-ping WANG
1
;
Guan-ling XU
1
;
Xin YAN
1
;
Xiao LI
1
;
Xiao XU
1
;
Xiang LI
1
;
Si-qi SUN
1
;
Xue-yang REN
1
;
Xiao-yun LIU
1
;
Yu WANG
1
;
Gai-mei ZHE
1
Author Information
- Publication Type:Research Article
- Keywords: herbal pair; Curcumae Rhizoma; Sparganii Rhizoma; phenols; organic acids; UPLC-LTQ-Orbitrap-MSn
- From: Chinese Journal of Experimental Traditional Medical Formulae 2020;26(23):171-179
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate constituents containing phenolic hydroxyl or carboxylic acid （excluding diarylheptanoids） from Curcumae Rhizoma and Sparganii Rhizoma herbal pair and single herb. Method:Multiple chromatographic separation techniques， including silica gel，MCI gel and Sephadex LH-20 gel， were employed to isolate and purify the compounds. Their structures were identified by means of the nuclear magnetic resonance （NMR），mass spectrometry （MS） and physicochemical properties. Constituents were quickly analyzed by UPLC-LTQ-Orbitrap-MS
n ，and scanned in positive and negative ion modes. Result:These compounds were determined as trans-p -hydroxycinnamic acid（1 ），vanillic acid（2 ），protocatechuic acid（3 ），fumalic acid（4 ），succinic acid（5 ），succinic acid monomethyl ester（6 ），docosanoic acid（7 ），azelaic acid（8 ） andp -hydroxybenzaldehyde（9 ）. Forty compounds were speculated by comparing mass spectrometry data，retention times of some compounds and reference materials，including 14 phenols and 26 organic acids. Among the compounds of herbal pair，8 phenols and 22 organic acids in Sparganii Rhizoma as well as
